Diadenosine tetraphosphate-induced inhibition of ATP-sensitive K+ channels in patches excised from ventricular myocytes

Aleksandar Jovanovic, Andre Terzic

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Diadenosine 5',5'-P1,P4-tetraphosphate (Ap4A) has been termed 'alarmone' due to its role in intracellular signaling during metabolic stress. It is not known whether Ap4A could modulate ATP-sensitive K+ (K(ATP)) channels, a family of channels regulated by the metabolic status of a cell. We applied the single-channel patch-clamp technique to measure the effect of Ap4A on K(ATP) channels. When applied to the intracellular side of patches, excised from guinea-pig ventricular myocytes, Ap4A inhibited K(ATP) channel activity, in a reversible and concentration-dependent (half-maximal concentration ~17 μM) manner. We conclude that Ap4A, a naturally occurring diadenosine polyphosphate, is actually an inhibitor of the myocardial K(ATP) channel.

Original languageEnglish (US)
Pages (from-to)233-235
Number of pages3
JournalBritish Journal of Pharmacology
Volume117
Issue number2
DOIs
StatePublished - 1996

Keywords

  • ATP-sensitive K channel
  • Alarmone
  • Cardiomyocyte
  • Channel gating
  • Diadenosine 5',5'-P,P-tetraphosphate
  • Diadenosine polyphosphate
  • Guinea-pig
  • Heart

ASJC Scopus subject areas

  • Pharmacology

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