Development of gene-based therapies for cartilage repair

Glyn Palmer, Arnulf Pascher, Elvire Gouze, Jean Noel Gouze, Oliver Betz, Myron Spector, Paul D. Robbins, Christopher H. Evans, Steven C. Ghivizzani

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

Articular cartilage is particularly vulnerable to injury and degenerative conditions, and has a limited capacity for self-repair. Although current clinical procedures cannot restore a normal articular surface, there are a growing number of proteins that may be used to augment a repair process, or protect cartilage from degeneration. Because proteins are often difficult to administer effectively, gene therapy approaches are being developed to provide their sustained synthesis at sites of injury or disease. To promote cartilage repair, cDNAs can be targeted to synovium, or cartilage. Gene transfer to the synovium is generally considered more suitable for chondroprotective therapies that rely on expression of large amounts of anti-inflammatory mediators. The delivery of genes to cartilage defects to promote enhanced repair can be performed by either direct administration of gene delivery vectors, or by implantation of genetically modified chondrogenic cells. Variations of these methods have been used to demonstrate that exogenous cDNAs encoding growth factors can be delivered locally to sites of cartilage damage where they are expressed at physiologically relevant levels. Data is beginning to emerge that suggests that delivery and expression of these genes can influence a repair response toward the synthesis of normal articular cartilage in vivo. This article reviews the current status of gene delivery for cartilage healing and presents some of the remaining challenges.

Original languageEnglish (US)
Pages (from-to)259-273
Number of pages15
JournalCritical Reviews in Eukaryotic Gene Expression
Volume12
Issue number4
DOIs
StatePublished - Dec 1 2002

Keywords

  • Cartilage
  • Chondrocyte
  • Gene therapy
  • Growth factor
  • Matrix
  • Stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Palmer, G., Pascher, A., Gouze, E., Gouze, J. N., Betz, O., Spector, M., Robbins, P. D., Evans, C. H., & Ghivizzani, S. C. (2002). Development of gene-based therapies for cartilage repair. Critical Reviews in Eukaryotic Gene Expression, 12(4), 259-273. https://doi.org/10.1615/CritRevEukaryotGeneExpr.v12.i4.20