Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Baskar Balakrishnan, David Luckey, Eric Marietta, Melissa Karau, Robin Patel, Joseph A Murray, Veena D Taneja

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 103 colony-forming units (CFU) in the gut.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalAnaerobe
Volume48
DOIs
StatePublished - Dec 1 2017

Fingerprint

Prevotella
Real-Time Polymerase Chain Reaction
rRNA Genes
Stem Cells

Keywords

  • Anaerobe
  • Gut microbiome
  • Prevotella histicola
  • qPCR
  • Real-time PCR quantification

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Development of a real-time PCR method for quantification of Prevotella histicola from the gut. / Balakrishnan, Baskar; Luckey, David; Marietta, Eric; Karau, Melissa; Patel, Robin; Murray, Joseph A; Taneja, Veena D.

In: Anaerobe, Vol. 48, 01.12.2017, p. 37-41.

Research output: Contribution to journalArticle

Balakrishnan, Baskar ; Luckey, David ; Marietta, Eric ; Karau, Melissa ; Patel, Robin ; Murray, Joseph A ; Taneja, Veena D. / Development of a real-time PCR method for quantification of Prevotella histicola from the gut. In: Anaerobe. 2017 ; Vol. 48. pp. 37-41.
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