Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Baskar Balakrishnan; David Luckey; Eric Marietta; Melissa Karau; Robin Patel; Joseph Murray; Veena Taneja

doi:10.1016/j.anaerobe.2017.06.019

Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Baskar Balakrishnan, David Luckey, Eric Marietta, Melissa Karau, Robin Patel, Joseph Murray, Veena Taneja

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 10³ colony-forming units (CFU) in the gut.

Original language	English (US)
Pages (from-to)	37-41
Number of pages	5
Journal	Anaerobe
Volume	48
DOIs	https://doi.org/10.1016/j.anaerobe.2017.06.019
State	Published - Dec 2017

Keywords

Anaerobe
Gut microbiome
Prevotella histicola
Real-time PCR quantification
qPCR

ASJC Scopus subject areas

Microbiology
Infectious Diseases

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10.1016/j.anaerobe.2017.06.019

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title = "Development of a real-time PCR method for quantification of Prevotella histicola from the gut",

abstract = "We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 103 colony-forming units (CFU) in the gut.",

keywords = "Anaerobe, Gut microbiome, Prevotella histicola, Real-time PCR quantification, qPCR",

author = "Baskar Balakrishnan and David Luckey and Eric Marietta and Melissa Karau and Robin Patel and Joseph Murray and Veena Taneja",

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doi = "10.1016/j.anaerobe.2017.06.019",

language = "English (US)",

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AU - Balakrishnan, Baskar

AU - Luckey, David

AU - Marietta, Eric

AU - Karau, Melissa

AU - Patel, Robin

AU - Murray, Joseph

AU - Taneja, Veena

PY - 2017/12

Y1 - 2017/12

N2 - We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 103 colony-forming units (CFU) in the gut.

AB - We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 103 colony-forming units (CFU) in the gut.

KW - Anaerobe

KW - Gut microbiome

KW - Prevotella histicola

KW - Real-time PCR quantification

KW - qPCR

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EP - 41

JO - Anaerobe

JF - Anaerobe

ER -

Development of a real-time PCR method for quantification of Prevotella histicola from the gut

Abstract

Keywords

ASJC Scopus subject areas

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