Development and immunophenotyping of squamous cell carcinoma xenografts: Tools for translational immunology

Wei Li, Xioayu Zhang, Zhaorong Chen, Nancy Borson, Steve Voss, Schuyler Sanderson, Linda Murphy, Peter Wettstein, Scott E. Strome

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objectives/Hypothesis: The objectives of this study were to delineate methods for the development of primary squamous cell carcinoma (SCCHN) xenografts and to define human leukocyte antigen (HLA), melanoma-associated antigen (MAGE)-AS, and human papilloma virus (HPV) 16 antigenic expression in resultant cellular products. Study Design: Prospective experimental model. Methods: Freshly isolated SCCHN xenografts were established in nonobese diabetic/severe combined immunodeficiency (NOD/SCED) mice using a variety of methods. Resultant tumors were analyzed for expression patterns of HLA-A, MAGE-A3, and HPV 16. Appropriate controls were included to ensure the presence of human RNA Results: Three xenografts were successfully established and passaged in vivo. Characterization of the resultant products revealed that one was positive for HLA-A2 at both the DNA and protein levels. One of the tumor lines expressed MAGE-A3, whereas none expressed HPV 16. Conclusions: Freshly isolated SCCHN can be used to generate primary xenografts. Characterization of select patterns of protein expression in established xenografts is a precursor to the development of a mouse model for SCCHN using tumor bearing animals reconstituted with autologous patient leukocytes.

Original languageEnglish (US)
Pages (from-to)1154-1162
Number of pages9
JournalLaryngoscope
Volume115
Issue number7
DOIs
StatePublished - Jul 1 2005

Keywords

  • Costimulation
  • Peptides
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Li, W., Zhang, X., Chen, Z., Borson, N., Voss, S., Sanderson, S., Murphy, L., Wettstein, P., & Strome, S. E. (2005). Development and immunophenotyping of squamous cell carcinoma xenografts: Tools for translational immunology. Laryngoscope, 115(7), 1154-1162. https://doi.org/10.1097/01.MLG.0000165368.81032.E2