Detection of soluble forms of the β-amyloid precursor protein in human plasma

Marcia B. Podlisny, Andrew L. Mammen, Michael G. Schlossmacher, Mark R. Palmert, Steven G Younkin, Dennis J. Selkoe

Research output: Contribution to journalArticle

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Abstract

A ∼40-residue fragment of the β-amyloid precursor protein (APP) is progressively deposited in the extracellular spaces of brain and blood vessels in Alzheimer's disease (AD), Down's syndrome and aged normal subjects. Soluble, truncated forms of APP lacking the carboxyl terminus are normally secreted from cultured cells expressing this protein and are found in cerebrospinal fluid. Here, we report the detection of a similar soluble APP isoform in human plasma. This ∼125 kDa protein, which was isolated from plasma by Affi-Gel Blue chromatography or dialysis-induced precipitation, comigrates with the larger of the two major soluble APP forms present in spinal fluid and contains the Kunitz protease inhibitor insert. It thus derives from the APP751 and APP770 precursors; a soluble form of APP695 has not yet been detected in plasma. The ∼125 kDa plasma form lacks the C-terminal region and is unlikely to serve as a precursor for the β-protein that forms the amyloid in AD.

Original languageEnglish (US)
Pages (from-to)1094-1101
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number3
DOIs
StatePublished - Mar 30 1990
Externally publishedYes

Fingerprint

Plasma (human)
Amyloid beta-Protein Precursor
Cibacron Blue F 3GA
Plasmas
Cerebrospinal fluid
Alzheimer Disease
Protein Precursors
Die casting inserts
Dialysis
Blood vessels
Chromatography
Protease Inhibitors
Amyloid
Brain
Protein Isoforms
Extracellular Space
Proteins
Down Syndrome
Cells
Gel Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection of soluble forms of the β-amyloid precursor protein in human plasma. / Podlisny, Marcia B.; Mammen, Andrew L.; Schlossmacher, Michael G.; Palmert, Mark R.; Younkin, Steven G; Selkoe, Dennis J.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 3, 30.03.1990, p. 1094-1101.

Research output: Contribution to journalArticle

Podlisny, Marcia B. ; Mammen, Andrew L. ; Schlossmacher, Michael G. ; Palmert, Mark R. ; Younkin, Steven G ; Selkoe, Dennis J. / Detection of soluble forms of the β-amyloid precursor protein in human plasma. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 167, No. 3. pp. 1094-1101.
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