Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA

Huy Quang Quach; Inna G. Ovsyannikova; Gregory A. Poland; Richard B. Kennedy

doi:10.1016/j.jim.2022.113275

Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA

Huy Quang Quach, Inna G. Ovsyannikova, Gregory A. Poland, Richard B. Kennedy

General Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.

Original language	English (US)
Article number	113275
Journal	Journal of Immunological Methods
Volume	505
DOIs	https://doi.org/10.1016/j.jim.2022.113275
State	Published - Jun 2022

Keywords

COVID-19 vaccine
ELISA
Golden Syrian hamsters
Peptide-based vaccine
SARS-CoV-2

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jim.2022.113275

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title = "Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA",

abstract = "Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.",

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AU - Poland, Gregory A.

AU - Kennedy, Richard B.

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AB - Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.

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KW - Peptide-based vaccine

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Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA

Abstract

Keywords

ASJC Scopus subject areas

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