Detection of hepatitis G virus replication sites by using highly strand- specific Tth-based reverse transcriptase PCR

Tomasz Laskus, Marek Radkowski, Lian Fu Wang, Hugo Vargas, Jorge Rakela

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The replication sites of the recently discovered hepatitis G virus (HGV) remain unknown. Using highly strand-specific Tth-based reverse transcriptase PCR, we searched for the presence of viral RNA negative strand in multiple autopsy tissues from four patients with AIDS and in peripheral blood mononuclear cells from six other human immunodeficiency virus-positive patients. Negative-strand HGV RNA was detected in three of four bone marrow samples, in two of two spleen samples, and in one of four liver tissue samples. However, the specific cellular site of replication within the positive tissues was not determined. This study does not support HGV as a primary hepatotropic virus.

Original languageEnglish (US)
Pages (from-to)3072-3075
Number of pages4
JournalJournal of virology
Volume72
Issue number4
DOIs
StatePublished - Apr 1998

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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