Detection of elevated signaling amino acids in human diabetic vitreous by rapid capillary electrophoresis

Miao Jen Lu, Jose S Pulido, Colin A. McCannel, Jose E. Pulido, R. Mark Hatfield, Robert F. Dundervill, Scott A. Shippy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Elevated glutamate is implicated in the pathology of PDR. The ability to rapidly assess the glutamate and amino acid content of vitreous provides a more complete picture of the chemical changes occurring at the diabetic retina and may lead to a better understanding of the pathology of PDR. Vitreous humor was collected following vitrectomies of patients with PDR and control conditions of macular hole or epiretinal membrane. A capillary electrophoresis method was developed to quantify glutamate and arginine. The analysis is relatively fast (<6 minutes) and utilizes a poly(ethylene)oxide and sodium dodecylsulfate run buffer. Both amino acid levels show significant increases in PDR patients versus controls and are comparable to other reports. The levels of vitreal glutamate vary inversely with the degree of observed hemorrhage. The results demonstrate a rapid method for assessment of a number of amino acids to characterize the chemical changes at the diabetic retina to better understand tissue changes and potentially identify new treatments.

Original languageEnglish (US)
Article number39765
JournalExperimental Diabesity Research
Volume2007
DOIs
StatePublished - 2007

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Capillary Electrophoresis
Glutamic Acid
arginine glutamate
Amino Acids
Retina
Pathology
Epiretinal Membrane
Ethylene Oxide
Vitreous Body
Retinal Perforations
Vitrectomy
Buffers
Sodium
Hemorrhage
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

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Detection of elevated signaling amino acids in human diabetic vitreous by rapid capillary electrophoresis. / Lu, Miao Jen; Pulido, Jose S; McCannel, Colin A.; Pulido, Jose E.; Hatfield, R. Mark; Dundervill, Robert F.; Shippy, Scott A.

In: Experimental Diabesity Research, Vol. 2007, 39765, 2007.

Research output: Contribution to journalArticle

Lu, Miao Jen ; Pulido, Jose S ; McCannel, Colin A. ; Pulido, Jose E. ; Hatfield, R. Mark ; Dundervill, Robert F. ; Shippy, Scott A. / Detection of elevated signaling amino acids in human diabetic vitreous by rapid capillary electrophoresis. In: Experimental Diabesity Research. 2007 ; Vol. 2007.
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