Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy

Scott Koenig, Howard E. Gendelman, Jan M. Orenstein, Mauro C. Dal Canto, Gholam H. Pezeshkpour, Margaret Yungbluth, Frank Janotta, Allen Jr. Aksamit, Malcolm A. Martin, Anthony S. Fauci

Research output: Contribution to journalArticle

1280 Citations (Scopus)

Abstract

One of the common neurological complications in patients with the acquired immune deficiency syndrome (AIDS) is a subacute encephalopathy with progressive dementia. By using the techniques of cocultivation for virus isolation, in situ hybridization, immunocytochemistry, and transmission electron microscopy, the identity of an important cell type that supports replication of the AIDS retrovirus in brain tissue was determined in two affected individuals. These cells were mononucleated and multinucleated macrophages that actively synthesized viral RNA and produced progeny virions in the brains of the patients. Infected brain macrophages may serve as a reservoir for virus and as a vehicle for viral dissemination in the infected host.

Original languageEnglish (US)
Pages (from-to)1089-1093
Number of pages5
JournalScience
Volume233
Issue number4768
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Brain Diseases
Acquired Immunodeficiency Syndrome
Macrophages
HIV
Brain
Viruses
Viral RNA
Retroviridae
Coculture Techniques
Transmission Electron Microscopy
Virion
In Situ Hybridization
Dementia
Immunohistochemistry

ASJC Scopus subject areas

  • General

Cite this

Koenig, S., Gendelman, H. E., Orenstein, J. M., Dal Canto, M. C., Pezeshkpour, G. H., Yungbluth, M., ... Fauci, A. S. (1986). Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. Science, 233(4768), 1089-1093. https://doi.org/10.1126/science.3016903

Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. / Koenig, Scott; Gendelman, Howard E.; Orenstein, Jan M.; Dal Canto, Mauro C.; Pezeshkpour, Gholam H.; Yungbluth, Margaret; Janotta, Frank; Aksamit, Allen Jr.; Martin, Malcolm A.; Fauci, Anthony S.

In: Science, Vol. 233, No. 4768, 01.01.1986, p. 1089-1093.

Research output: Contribution to journalArticle

Koenig, S, Gendelman, HE, Orenstein, JM, Dal Canto, MC, Pezeshkpour, GH, Yungbluth, M, Janotta, F, Aksamit, AJ, Martin, MA & Fauci, AS 1986, 'Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy', Science, vol. 233, no. 4768, pp. 1089-1093. https://doi.org/10.1126/science.3016903
Koenig S, Gendelman HE, Orenstein JM, Dal Canto MC, Pezeshkpour GH, Yungbluth M et al. Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. Science. 1986 Jan 1;233(4768):1089-1093. https://doi.org/10.1126/science.3016903
Koenig, Scott ; Gendelman, Howard E. ; Orenstein, Jan M. ; Dal Canto, Mauro C. ; Pezeshkpour, Gholam H. ; Yungbluth, Margaret ; Janotta, Frank ; Aksamit, Allen Jr. ; Martin, Malcolm A. ; Fauci, Anthony S. / Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. In: Science. 1986 ; Vol. 233, No. 4768. pp. 1089-1093.
@article{cb27e99c3e684d59910eaf790f210426,
title = "Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy",
abstract = "One of the common neurological complications in patients with the acquired immune deficiency syndrome (AIDS) is a subacute encephalopathy with progressive dementia. By using the techniques of cocultivation for virus isolation, in situ hybridization, immunocytochemistry, and transmission electron microscopy, the identity of an important cell type that supports replication of the AIDS retrovirus in brain tissue was determined in two affected individuals. These cells were mononucleated and multinucleated macrophages that actively synthesized viral RNA and produced progeny virions in the brains of the patients. Infected brain macrophages may serve as a reservoir for virus and as a vehicle for viral dissemination in the infected host.",
author = "Scott Koenig and Gendelman, {Howard E.} and Orenstein, {Jan M.} and {Dal Canto}, {Mauro C.} and Pezeshkpour, {Gholam H.} and Margaret Yungbluth and Frank Janotta and Aksamit, {Allen Jr.} and Martin, {Malcolm A.} and Fauci, {Anthony S.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1126/science.3016903",
language = "English (US)",
volume = "233",
pages = "1089--1093",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4768",

}

TY - JOUR

T1 - Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy

AU - Koenig, Scott

AU - Gendelman, Howard E.

AU - Orenstein, Jan M.

AU - Dal Canto, Mauro C.

AU - Pezeshkpour, Gholam H.

AU - Yungbluth, Margaret

AU - Janotta, Frank

AU - Aksamit, Allen Jr.

AU - Martin, Malcolm A.

AU - Fauci, Anthony S.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - One of the common neurological complications in patients with the acquired immune deficiency syndrome (AIDS) is a subacute encephalopathy with progressive dementia. By using the techniques of cocultivation for virus isolation, in situ hybridization, immunocytochemistry, and transmission electron microscopy, the identity of an important cell type that supports replication of the AIDS retrovirus in brain tissue was determined in two affected individuals. These cells were mononucleated and multinucleated macrophages that actively synthesized viral RNA and produced progeny virions in the brains of the patients. Infected brain macrophages may serve as a reservoir for virus and as a vehicle for viral dissemination in the infected host.

AB - One of the common neurological complications in patients with the acquired immune deficiency syndrome (AIDS) is a subacute encephalopathy with progressive dementia. By using the techniques of cocultivation for virus isolation, in situ hybridization, immunocytochemistry, and transmission electron microscopy, the identity of an important cell type that supports replication of the AIDS retrovirus in brain tissue was determined in two affected individuals. These cells were mononucleated and multinucleated macrophages that actively synthesized viral RNA and produced progeny virions in the brains of the patients. Infected brain macrophages may serve as a reservoir for virus and as a vehicle for viral dissemination in the infected host.

UR - http://www.scopus.com/inward/record.url?scp=0022522203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022522203&partnerID=8YFLogxK

U2 - 10.1126/science.3016903

DO - 10.1126/science.3016903

M3 - Article

C2 - 3016903

AN - SCOPUS:0022522203

VL - 233

SP - 1089

EP - 1093

JO - Science

JF - Science

SN - 0036-8075

IS - 4768

ER -