Defensive perimeter in the central nervous system: Predominance of astrocytes and astrogliosis during recovery from varicella-zoster virus encephalitis

John E. Carpenter, Amy C. Clayton, Kevin C. Halling, Daniel J. Bonthius, Erin M. Buckingham, Wallen Jackson, Steven M. Dotzler, J. Patrick Card, Lynn W. Enquist, Charles Grose

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Varicella-zoster virus (VZV) is a highly neurotropic virus that can cause infections in both the peripheral nervous system and the central nervous system. Several studies of VZV reactivation in the peripheral nervous system (herpes zoster) have been published, while exceedingly few investigations have been carried out in a human brain. Notably, there is no animal model for VZV infection of the central nervous system. In this report, we characterized the cellular environment in the temporal lobe of a human subject who recovered from focal VZV encephalitis. The approach included not only VZV DNA/RNA analyses but also a delineation of infected cell types (neurons, microglia, oligodendrocytes, and astrocytes). The average VZV genome copy number per cell was 5. Several VZV regulatory and structural gene transcripts and products were detected. When colocalization studies were performed to determine which cell types harbored the viral proteins, the majority of infected cells were astrocytes, including aggregates of astrocytes. Evidence of syncytium formation within the aggregates included the continuity of cytoplasm positive for the VZV glycoprotein H (gH) fusion-complex protein within a cellular profile with as many as 80 distinct nuclei. As with other causes of brain injury, these results suggested that astrocytes likely formed a defensive perimeter around foci of VZV infection (astrogliosis). Because of the rarity of brain samples from living humans with VZV encephalitis, we compared our VZV results with those found in a rat encephalitis model following infection with the closely related pseudorabies virus and observed similar perimeters of gliosis.

Original languageEnglish (US)
Pages (from-to)379-391
Number of pages13
JournalJournal of Virology
Volume90
Issue number1
DOIs
StatePublished - 2016

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Varicella Zoster Encephalitis
Human herpesvirus 3
Human Herpesvirus 3
astrocytes
encephalitis
Astrocytes
central nervous system
Central Nervous System
peripheral nervous system
Peripheral Nervous System
protein aggregates
Virus Diseases
brain
infection
cells
Suid Herpesvirus 1
Gliosis
Suid herpesvirus 1
structural genes
viral proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Carpenter, J. E., Clayton, A. C., Halling, K. C., Bonthius, D. J., Buckingham, E. M., Jackson, W., ... Grose, C. (2016). Defensive perimeter in the central nervous system: Predominance of astrocytes and astrogliosis during recovery from varicella-zoster virus encephalitis. Journal of Virology, 90(1), 379-391. https://doi.org/10.1128/JVI.02389-15

Defensive perimeter in the central nervous system : Predominance of astrocytes and astrogliosis during recovery from varicella-zoster virus encephalitis. / Carpenter, John E.; Clayton, Amy C.; Halling, Kevin C.; Bonthius, Daniel J.; Buckingham, Erin M.; Jackson, Wallen; Dotzler, Steven M.; Card, J. Patrick; Enquist, Lynn W.; Grose, Charles.

In: Journal of Virology, Vol. 90, No. 1, 2016, p. 379-391.

Research output: Contribution to journalArticle

Carpenter, JE, Clayton, AC, Halling, KC, Bonthius, DJ, Buckingham, EM, Jackson, W, Dotzler, SM, Card, JP, Enquist, LW & Grose, C 2016, 'Defensive perimeter in the central nervous system: Predominance of astrocytes and astrogliosis during recovery from varicella-zoster virus encephalitis', Journal of Virology, vol. 90, no. 1, pp. 379-391. https://doi.org/10.1128/JVI.02389-15
Carpenter, John E. ; Clayton, Amy C. ; Halling, Kevin C. ; Bonthius, Daniel J. ; Buckingham, Erin M. ; Jackson, Wallen ; Dotzler, Steven M. ; Card, J. Patrick ; Enquist, Lynn W. ; Grose, Charles. / Defensive perimeter in the central nervous system : Predominance of astrocytes and astrogliosis during recovery from varicella-zoster virus encephalitis. In: Journal of Virology. 2016 ; Vol. 90, No. 1. pp. 379-391.
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