Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells

Jeong Hwan Kim, Soo Jin Jeong, Hee Young Kwon, Sang Yoon Park, Hyo Jung Lee, Hyo Jeong Lee, John C Lieske, Sung Hoon Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Adverse effects, nephrotoxicity and hepatotoxicity, of anticancer drugs such as cisplatin have limited the usage for cancer therapy. Therefore, development or identification of supplement agents in anticancer drugs is attractive to reduce side effects and enhance antitumor activity. Here, we found that decursin isolated from Angelica gigas showed protective effects of cisplatin-induced damage in normal human primary renal epithelial cells (HRCs). We found that decursin significantly blocked cisplatin-induced cytotoxicity by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2/7-tetrazolium-5-carboxanilide (XTT) assay in HRCs. Further, we found that decursin inhibited sub-G1 and cell death by suppression of cleavage of caspase-3, -9 and poly(ADP-ribose) polymerase (PARP) induced by cisplatin treatment in HRCs. Importantly, decursin effectively restored the activities of Cu/Zn superoxide dismutase (SOD), catalase and glutathione peroxidase in cisplatin-treated HRCs. Taken together, our findings demonstrate that decurcin prevents cisplatin-induced cytotoxicity and apoptosis through the activation of antioxidant enzymes in HRCs and suggest further that combination of decursin might suppressed adverse effects of anticancer drugs in cancer patients.

Original languageEnglish (US)
Pages (from-to)1279-1284
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume33
Issue number8
DOIs
StatePublished - 2010

Fingerprint

Cisplatin
Antioxidants
Epithelial Cells
Apoptosis
Kidney
Enzymes
Angelica
Pharmaceutical Preparations
Enzyme Activation
Poly(ADP-ribose) Polymerases
Caspase 9
Glutathione Peroxidase
Caspase 3
Antineoplastic Agents
Catalase
decursin
Neoplasms
Cell Death
Therapeutics

Keywords

  • Antioxidant enzyme
  • Apoptosis
  • Cisplatin
  • Decursin
  • Renal epithelial cell

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells. / Kim, Jeong Hwan; Jeong, Soo Jin; Kwon, Hee Young; Park, Sang Yoon; Lee, Hyo Jung; Lee, Hyo Jeong; Lieske, John C; Kim, Sung Hoon.

In: Biological and Pharmaceutical Bulletin, Vol. 33, No. 8, 2010, p. 1279-1284.

Research output: Contribution to journalArticle

Kim, Jeong Hwan ; Jeong, Soo Jin ; Kwon, Hee Young ; Park, Sang Yoon ; Lee, Hyo Jung ; Lee, Hyo Jeong ; Lieske, John C ; Kim, Sung Hoon. / Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells. In: Biological and Pharmaceutical Bulletin. 2010 ; Vol. 33, No. 8. pp. 1279-1284.
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