Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells

Jeong Hwan Kim, Soo Jin Jeong, Hee Young Kwon, Sang Yoon Park, Hyo Jung Lee, Hyo Jeong Lee, John Charles Lieske, Sung Hoon Kim

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Adverse effects, nephrotoxicity and hepatotoxicity, of anticancer drugs such as cisplatin have limited the usage for cancer therapy. Therefore, development or identification of supplement agents in anticancer drugs is attractive to reduce side effects and enhance antitumor activity. Here, we found that decursin isolated from Angelica gigas showed protective effects of cisplatin-induced damage in normal human primary renal epithelial cells (HRCs). We found that decursin significantly blocked cisplatin-induced cytotoxicity by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2/7-tetrazolium-5-carboxanilide (XTT) assay in HRCs. Further, we found that decursin inhibited sub-G1 and cell death by suppression of cleavage of caspase-3, -9 and poly(ADP-ribose) polymerase (PARP) induced by cisplatin treatment in HRCs. Importantly, decursin effectively restored the activities of Cu/Zn superoxide dismutase (SOD), catalase and glutathione peroxidase in cisplatin-treated HRCs. Taken together, our findings demonstrate that decurcin prevents cisplatin-induced cytotoxicity and apoptosis through the activation of antioxidant enzymes in HRCs and suggest further that combination of decursin might suppressed adverse effects of anticancer drugs in cancer patients.

Original languageEnglish (US)
Pages (from-to)1279-1284
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume33
Issue number8
DOIs
StatePublished - Sep 3 2010

Keywords

  • Antioxidant enzyme
  • Apoptosis
  • Cisplatin
  • Decursin
  • Renal epithelial cell

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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