Decreased Osteogenic Activity of Mesenchymal Stem Cells in Patients With Corticosteroid-Induced Osteonecrosis of the Femoral Head

Matthew T. Houdek, Cody C. Wyles, Benjamin D. Packard, Andre Terzic, Atta Behfar, Rafael J. Sierra

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Osteonecrosis (ON) of the femoral head occurs when cells of trabecular bone spontaneously die. Mesenchymal stem cells (MSCs) have been introduced into the femoral head in an attempt to halt progression of the disease. The purpose of this study was to functionally compare MSCs in patients with ON of the femoral head with patients without. Mesenchymal stem cells were isolated from 20 patients with corticosteroid-induced ON and 10 controls without. Colony-forming unit and proliferation assays were used to assess MSC proliferation. Mesenchymal stem cells were differentiated into bone, fat, and cartilage. Functional assays were used to quantify the differentiation capacity. Control MSCs demonstrated greater cellular growth potential and improved ability to differentiate into bone. The decreased ability to differentiate into bone may be a reason why patients treated with autologous MSC infusion fail regenerative treatment strategies and progress to collapse.

Original languageEnglish (US)
JournalJournal of Arthroplasty
DOIs
StateAccepted/In press - Jul 14 2015

Fingerprint

Osteonecrosis
Thigh
Mesenchymal Stromal Cells
Adrenal Cortex Hormones
Bone and Bones
Colony-Forming Units Assay
Cartilage
Disease Progression
Fats
Cell Proliferation
Growth

Keywords

  • Avascular necrosis
  • Femoral head
  • Osteonecrosis
  • Regenerative medicine
  • Stem cells

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Decreased Osteogenic Activity of Mesenchymal Stem Cells in Patients With Corticosteroid-Induced Osteonecrosis of the Femoral Head. / Houdek, Matthew T.; Wyles, Cody C.; Packard, Benjamin D.; Terzic, Andre; Behfar, Atta; Sierra, Rafael J.

In: Journal of Arthroplasty, 14.07.2015.

Research output: Contribution to journalArticle

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