Cytotoxic t cells and granzyme b associated with improved colorectal cancer survival in a prospective cohort of older women

Anna E. Prizment, Robert A. Vierkant, Thomas Christopher Smyrk, Lori S. Tillmans, Heather H. Nelson, Charles F. Lynch, Thomas Pengo, Stephen N Thibodeau, Timothy R. Church, James R Cerhan, Kristin E. Anderson, Paul John Limburg

Research output: Contribution to journalArticle

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Abstract

Background: Host immune response may predict the course of colorectal cancer. We examined the survival of 468 colorectal cancer patients associated with two tumor-infiltrating immune biomarkers, the number of cytotoxic T lymphocytes (CTLs), and the activated CTLs, as reflected by the number of cells expressing granzyme B (GZMB) in the prospective Iowa Women's Health Study. Methods: Using paraffin-embedded tissue samples, we constructed and immunostained tumor microarrays with CD8 (for CTL) and GZMB antibodies. We scored CTL and GZMB densities in tumor epithelial and stromal tissues and also created a composite score for each biomarker (sum of the scores across tissue compartments). Cox regression estimated the HR and 95% confidence intervals (CI) for all-cause and colorectal cancer-specific death associated with each composite score. Results: CTL and GZMB composite scores were positively correlated (r 0.65) and each biomarker was inversely correlated with stage at diagnosis. Both composite scores were higher in proximal colon tumors and tumors characterized by MSI-high, CIMP-high, or BRAF mutation status. HRs (95% CI) were 0.53 (0.38-0.75; Ptrend 0.0004) and 0.66 (0.51-0.86; Ptrend 0.002) for all-cause death, respectively, and 0.30 (0.18-0.51; Ptrend < 0.0001) and 0.41 (0.27-0.63; Ptrend < 0.0001) for colorectal cancer-related death, respectively. Including CTL and GZMB scores simultaneously in the model significantly improved the predictive performance of the models for all-cause and colorectal cancer-related death. Conclusions: Higher tumor infiltration with CTL and GZMB cells is associated with improved all-cause and cancer-specific survival of colorectal cancer patients. Impact: Both the number of CTLs and GZMB appear to be useful prognostic factors in colorectal cancer, irrespective of stage.

Original languageEnglish (US)
Pages (from-to)622-631
Number of pages10
JournalCancer Epidemiology Biomarkers and Prevention
Volume26
Issue number4
DOIs
StatePublished - Apr 1 2017

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Granzymes
Cytotoxic T-Lymphocytes
Colorectal Neoplasms
Survival
Neoplasms
Biomarkers
Confidence Intervals
Women's Health
Paraffin
Cause of Death
Colon
B-Lymphocytes
Epithelium
Cell Count
Mutation
Antibodies

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Cytotoxic t cells and granzyme b associated with improved colorectal cancer survival in a prospective cohort of older women. / Prizment, Anna E.; Vierkant, Robert A.; Smyrk, Thomas Christopher; Tillmans, Lori S.; Nelson, Heather H.; Lynch, Charles F.; Pengo, Thomas; Thibodeau, Stephen N; Church, Timothy R.; Cerhan, James R; Anderson, Kristin E.; Limburg, Paul John.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 26, No. 4, 01.04.2017, p. 622-631.

Research output: Contribution to journalArticle

Prizment, Anna E. ; Vierkant, Robert A. ; Smyrk, Thomas Christopher ; Tillmans, Lori S. ; Nelson, Heather H. ; Lynch, Charles F. ; Pengo, Thomas ; Thibodeau, Stephen N ; Church, Timothy R. ; Cerhan, James R ; Anderson, Kristin E. ; Limburg, Paul John. / Cytotoxic t cells and granzyme b associated with improved colorectal cancer survival in a prospective cohort of older women. In: Cancer Epidemiology Biomarkers and Prevention. 2017 ; Vol. 26, No. 4. pp. 622-631.
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abstract = "Background: Host immune response may predict the course of colorectal cancer. We examined the survival of 468 colorectal cancer patients associated with two tumor-infiltrating immune biomarkers, the number of cytotoxic T lymphocytes (CTLs), and the activated CTLs, as reflected by the number of cells expressing granzyme B (GZMB) in the prospective Iowa Women's Health Study. Methods: Using paraffin-embedded tissue samples, we constructed and immunostained tumor microarrays with CD8 (for CTL) and GZMB antibodies. We scored CTL and GZMB densities in tumor epithelial and stromal tissues and also created a composite score for each biomarker (sum of the scores across tissue compartments). Cox regression estimated the HR and 95{\%} confidence intervals (CI) for all-cause and colorectal cancer-specific death associated with each composite score. Results: CTL and GZMB composite scores were positively correlated (r 0.65) and each biomarker was inversely correlated with stage at diagnosis. Both composite scores were higher in proximal colon tumors and tumors characterized by MSI-high, CIMP-high, or BRAF mutation status. HRs (95{\%} CI) were 0.53 (0.38-0.75; Ptrend 0.0004) and 0.66 (0.51-0.86; Ptrend 0.002) for all-cause death, respectively, and 0.30 (0.18-0.51; Ptrend < 0.0001) and 0.41 (0.27-0.63; Ptrend < 0.0001) for colorectal cancer-related death, respectively. Including CTL and GZMB scores simultaneously in the model significantly improved the predictive performance of the models for all-cause and colorectal cancer-related death. Conclusions: Higher tumor infiltration with CTL and GZMB cells is associated with improved all-cause and cancer-specific survival of colorectal cancer patients. Impact: Both the number of CTLs and GZMB appear to be useful prognostic factors in colorectal cancer, irrespective of stage.",
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T1 - Cytotoxic t cells and granzyme b associated with improved colorectal cancer survival in a prospective cohort of older women

AU - Prizment, Anna E.

AU - Vierkant, Robert A.

AU - Smyrk, Thomas Christopher

AU - Tillmans, Lori S.

AU - Nelson, Heather H.

AU - Lynch, Charles F.

AU - Pengo, Thomas

AU - Thibodeau, Stephen N

AU - Church, Timothy R.

AU - Cerhan, James R

AU - Anderson, Kristin E.

AU - Limburg, Paul John

PY - 2017/4/1

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N2 - Background: Host immune response may predict the course of colorectal cancer. We examined the survival of 468 colorectal cancer patients associated with two tumor-infiltrating immune biomarkers, the number of cytotoxic T lymphocytes (CTLs), and the activated CTLs, as reflected by the number of cells expressing granzyme B (GZMB) in the prospective Iowa Women's Health Study. Methods: Using paraffin-embedded tissue samples, we constructed and immunostained tumor microarrays with CD8 (for CTL) and GZMB antibodies. We scored CTL and GZMB densities in tumor epithelial and stromal tissues and also created a composite score for each biomarker (sum of the scores across tissue compartments). Cox regression estimated the HR and 95% confidence intervals (CI) for all-cause and colorectal cancer-specific death associated with each composite score. Results: CTL and GZMB composite scores were positively correlated (r 0.65) and each biomarker was inversely correlated with stage at diagnosis. Both composite scores were higher in proximal colon tumors and tumors characterized by MSI-high, CIMP-high, or BRAF mutation status. HRs (95% CI) were 0.53 (0.38-0.75; Ptrend 0.0004) and 0.66 (0.51-0.86; Ptrend 0.002) for all-cause death, respectively, and 0.30 (0.18-0.51; Ptrend < 0.0001) and 0.41 (0.27-0.63; Ptrend < 0.0001) for colorectal cancer-related death, respectively. Including CTL and GZMB scores simultaneously in the model significantly improved the predictive performance of the models for all-cause and colorectal cancer-related death. Conclusions: Higher tumor infiltration with CTL and GZMB cells is associated with improved all-cause and cancer-specific survival of colorectal cancer patients. Impact: Both the number of CTLs and GZMB appear to be useful prognostic factors in colorectal cancer, irrespective of stage.

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