Cytotoxic properties of Diospyros seychellarum extract

Eric J. Buenz, Brent A Bauer, Timothy J. Motley, Paul John Limburg

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

During a recent botanical expedition in the Seychelles archipelago we identified healers using Diospyros seychellarum as a tonic. Since this plant lacks any medicinal record in the current literature, we assessed the cytotoxic potential of D. seychellarum. Using Jurkat cells as a model system we show, by flow cytometry, that treatment with the leaf extract results in mitochondrial depolarization and subsequent loss of cellular membrane integrity. Additionally, by transmission electron microscopy, we show that treatment with the extract results in chromatin condensation, mitochondrial swelling, and loss of nuclear membrane integrity. Through these morphological and biochemical observations we concluded that the extract of Diospyros seychellarum is able to induce apoptosis. While it is difficult to extrapolate a potential pharmacologic function based on the ethnomedical use as a tonic, the ability of this extract to induce apoptosis warrants further investigation of the medicinal properties of this plant.

Original languageEnglish (US)
Pages (from-to)487-493
Number of pages7
JournalJournal of Toxicological Sciences
Volume32
Issue number5
DOIs
StatePublished - Dec 2007

    Fingerprint

Keywords

  • Apoptosis
  • Cell death
  • Cytometry
  • Mitochondria
  • Plant
  • Seychelles

ASJC Scopus subject areas

  • Toxicology

Cite this