Cyclosporine increases human immunodeficiency virus type 1 vector transduction of primary mouse cells

Josh A. Noser, Greg J. Towers, Ryuta Sakuma, Jean Maurice Dumont, Mary K.L. Collins, Yasuhiro Ikeda

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Murine primary cells are poorly permissive to human immunodeficiency virus type 1 (HIV-1) vector infection. Retroviral infectivity is influenced by dominant inhibitors such as TRIM5α. Sensitivity to TRIM5α is altered by interactions between cyclophilin A and the HIV-1 capsid. Here we demonstrate that competitive inhibitors of cyclophilins, cyclosporine or the related Debio-025, stimulate HIV-1 vector transduction of primary murine cells, including bone marrow and macrophages, up to 20-fold. Unexpectedly, the infectivity of an HIV-1 mutant or a simian lentivirus that does not recruit cyclophilin A is also stimulated by these drugs. We propose that cyclosporine and related compounds will be useful tools for experimental infection of murine primary cells. It is possible that HIV-1 infection of murine cells is inhibited by dominant factors related to immunophilins.

Original languageEnglish (US)
Pages (from-to)7769-7774
Number of pages6
JournalJournal of virology
Volume80
Issue number15
DOIs
StatePublished - Aug 2006

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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