Cyclic ADP-ribose stimulates sarcoplasmic reticulum calcium release in porcine coronary artery smooth muscle

Mathur S. Kannan, Alexis M. Fenton, Y. S. Prakash, Gary C. Sieck

Research output: Contribution to journalArticle

83 Scopus citations


Cyclic ADP-ribose (cADPR) was shown to induce calcium release from the endoplasmic reticulum via ryanodine-sensitive pathways. In smooth muscle, two pathways for calcium release from the sarcoplasmic reticulum (SR) have been previously demonstrated: D-myo-inositol 1,4,5-trisphosphate-gated and ryanodine-gated. However, evidence for cADPR as a regulator for SR Ca 2+ release in smooth muscle is lacking. We used permeabilized porcine coronary artery smooth muscle cells to directly examine the stimulation of SR Ca 2+ release by cADPR. The results provide direct evidence that cADPR stimulates SR Ca 2+ release and that this response is not inhibited by heparin, by depletion of the caffeine-sensitive Ca 2+ pool, or by blockade of ryanodine receptors. These results indicate a novel mechanism for Ca 2+ release from the SR of vascular smooth muscle.

Original languageEnglish (US)
Pages (from-to)H801-H806
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number2 39-2
StatePublished - Apr 15 1996



  • caffeine
  • dissociated cells
  • fluorescence imaging
  • heparin
  • ryanodine
  • β- escin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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