Cutting edge

Nuclear factor of activated T cells and AP-1 are insufficient for IL-2 promoter activation: Requirement for CD28 up- regulation of RE/AP

Virginia M Shapiro, Marianne Newton Mollenauer, Arthur Weiss

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

IL-2 gene transcription in T cells requires both TCR and costimulatory signals. IL-2 promoter activation in Jurkat T cells stimulated with superantigen presented by Raji B cells requires CD28 activation. The addition of rCTLA4Ig, which blocks CD28 binding to its ligand, to the cultures decreased IL-2 promoter activation by >80%. Interestingly, CTLA4Ig did not significantly inhibit the activation of either NF of activated T cells (NFAT) or AP-1 reporters. Therefore, activation of NFAT and AP-1 is insufficient for IL-2 promoter activation. In contrast, an RE/AP reporter was blocked by CTLA4Ig by >90%. Thus, the requirement for CD28 in IL-2 promoter activation appears to be due to RE/AP and not the NFAT or AP-1 sites. In addition, these data suggest that transcriptional activation of RE/AP is not mediated by NFAT, because activation of a NFAT reporter is not affected by the addition of CTLA4Ig.

Original languageEnglish (US)
Pages (from-to)6455-6458
Number of pages4
JournalJournal of Immunology
Volume161
Issue number12
StatePublished - Dec 15 1998
Externally publishedYes

Fingerprint

NFATC Transcription Factors
Transcription Factor AP-1
Interleukin-2
Up-Regulation
T-Lymphocytes
Superantigens
Jurkat Cells
Transcriptional Activation
B-Lymphocytes
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : Nuclear factor of activated T cells and AP-1 are insufficient for IL-2 promoter activation: Requirement for CD28 up- regulation of RE/AP. / Shapiro, Virginia M; Mollenauer, Marianne Newton; Weiss, Arthur.

In: Journal of Immunology, Vol. 161, No. 12, 15.12.1998, p. 6455-6458.

Research output: Contribution to journalArticle

@article{ef17fd2e507e4947af920c6980bc38d9,
title = "Cutting edge: Nuclear factor of activated T cells and AP-1 are insufficient for IL-2 promoter activation: Requirement for CD28 up- regulation of RE/AP",
abstract = "IL-2 gene transcription in T cells requires both TCR and costimulatory signals. IL-2 promoter activation in Jurkat T cells stimulated with superantigen presented by Raji B cells requires CD28 activation. The addition of rCTLA4Ig, which blocks CD28 binding to its ligand, to the cultures decreased IL-2 promoter activation by >80{\%}. Interestingly, CTLA4Ig did not significantly inhibit the activation of either NF of activated T cells (NFAT) or AP-1 reporters. Therefore, activation of NFAT and AP-1 is insufficient for IL-2 promoter activation. In contrast, an RE/AP reporter was blocked by CTLA4Ig by >90{\%}. Thus, the requirement for CD28 in IL-2 promoter activation appears to be due to RE/AP and not the NFAT or AP-1 sites. In addition, these data suggest that transcriptional activation of RE/AP is not mediated by NFAT, because activation of a NFAT reporter is not affected by the addition of CTLA4Ig.",
author = "Shapiro, {Virginia M} and Mollenauer, {Marianne Newton} and Arthur Weiss",
year = "1998",
month = "12",
day = "15",
language = "English (US)",
volume = "161",
pages = "6455--6458",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - Cutting edge

T2 - Nuclear factor of activated T cells and AP-1 are insufficient for IL-2 promoter activation: Requirement for CD28 up- regulation of RE/AP

AU - Shapiro, Virginia M

AU - Mollenauer, Marianne Newton

AU - Weiss, Arthur

PY - 1998/12/15

Y1 - 1998/12/15

N2 - IL-2 gene transcription in T cells requires both TCR and costimulatory signals. IL-2 promoter activation in Jurkat T cells stimulated with superantigen presented by Raji B cells requires CD28 activation. The addition of rCTLA4Ig, which blocks CD28 binding to its ligand, to the cultures decreased IL-2 promoter activation by >80%. Interestingly, CTLA4Ig did not significantly inhibit the activation of either NF of activated T cells (NFAT) or AP-1 reporters. Therefore, activation of NFAT and AP-1 is insufficient for IL-2 promoter activation. In contrast, an RE/AP reporter was blocked by CTLA4Ig by >90%. Thus, the requirement for CD28 in IL-2 promoter activation appears to be due to RE/AP and not the NFAT or AP-1 sites. In addition, these data suggest that transcriptional activation of RE/AP is not mediated by NFAT, because activation of a NFAT reporter is not affected by the addition of CTLA4Ig.

AB - IL-2 gene transcription in T cells requires both TCR and costimulatory signals. IL-2 promoter activation in Jurkat T cells stimulated with superantigen presented by Raji B cells requires CD28 activation. The addition of rCTLA4Ig, which blocks CD28 binding to its ligand, to the cultures decreased IL-2 promoter activation by >80%. Interestingly, CTLA4Ig did not significantly inhibit the activation of either NF of activated T cells (NFAT) or AP-1 reporters. Therefore, activation of NFAT and AP-1 is insufficient for IL-2 promoter activation. In contrast, an RE/AP reporter was blocked by CTLA4Ig by >90%. Thus, the requirement for CD28 in IL-2 promoter activation appears to be due to RE/AP and not the NFAT or AP-1 sites. In addition, these data suggest that transcriptional activation of RE/AP is not mediated by NFAT, because activation of a NFAT reporter is not affected by the addition of CTLA4Ig.

UR - http://www.scopus.com/inward/record.url?scp=0032534281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032534281&partnerID=8YFLogxK

M3 - Article

VL - 161

SP - 6455

EP - 6458

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -