Crystal structure of the nuclear matrix targeting signal of the transcription factor acute myelogenous leukemia-1/polyoma enhancer-binding protein 2αB/core binding factor α2

Lei Tang, Bo Guo, Amjad Javed, Je Yong Choi, Scott Hiebert, Jane B. Lian, Andre J van Wijnen, Janet L. Stein, Gary S. Stein, G. Wayne Zhou

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Transcription factors of the acute myelogenous leukemia (AML)/polyoma enhancer-binding protein (PEBP2α)/core-binding factor α (CBFA) class are key transactivators of tissue-specific genes of the hematopoietic and bone lineages. AML-1/PEBP2αB/CBFA2 proteins participating in transcription are associated with the nuclear matrix. This association is solely dependent on a highly conserved C-terminal protein segment, designated the nuclear matrix targeting signal (NMTS). The NMTS of AML-1 is physically distinct from the nuclear localization signal, operates autonomously, and supports transactivation. Our data indicate that the related AML-3 and AML-2 proteins are also targeted to the nuclear matrix in situ by analogous C-terminal domains. Here we report the first crystal structure of an NMTS in an AML-1 segment fused to glutathione S-transferase. The model of the NMTS consists of two loops connected by a flexible U-shaped peptide chain.

Original languageEnglish (US)
Pages (from-to)33580-33586
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number47
DOIs
StatePublished - Nov 19 1999
Externally publishedYes

Fingerprint

Core Binding Factors
Nuclear Matrix
Acute Myeloid Leukemia
Carrier Proteins
Transcription Factors
Crystal structure
Proteins
Trans-Activators
Transcription
Glutathione Transferase
Bone
Genes
Tissue
Nuclear Localization Signals
Peptides
Protein C
Transcriptional Activation
Bone and Bones

ASJC Scopus subject areas

  • Biochemistry

Cite this

Crystal structure of the nuclear matrix targeting signal of the transcription factor acute myelogenous leukemia-1/polyoma enhancer-binding protein 2αB/core binding factor α2. / Tang, Lei; Guo, Bo; Javed, Amjad; Choi, Je Yong; Hiebert, Scott; Lian, Jane B.; van Wijnen, Andre J; Stein, Janet L.; Stein, Gary S.; Zhou, G. Wayne.

In: Journal of Biological Chemistry, Vol. 274, No. 47, 19.11.1999, p. 33580-33586.

Research output: Contribution to journalArticle

Tang, Lei ; Guo, Bo ; Javed, Amjad ; Choi, Je Yong ; Hiebert, Scott ; Lian, Jane B. ; van Wijnen, Andre J ; Stein, Janet L. ; Stein, Gary S. ; Zhou, G. Wayne. / Crystal structure of the nuclear matrix targeting signal of the transcription factor acute myelogenous leukemia-1/polyoma enhancer-binding protein 2αB/core binding factor α2. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 47. pp. 33580-33586.
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