Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513

Ai Yu Gong, Rui Zhou, Guoku Hu, Jun Liu, Danuta Sosnowska, Kristen M. Drescher, Haidong Dong, Xian Ming Chen

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Abstract

Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

Original languageEnglish (US)
Pages (from-to)160-169
Number of pages10
JournalJournal of Infectious Diseases
Volume201
Issue number1
DOIs
StatePublished - Jan 1 2010

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Gong, A. Y., Zhou, R., Hu, G., Liu, J., Sosnowska, D., Drescher, K. M., Dong, H., & Chen, X. M. (2010). Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. Journal of Infectious Diseases, 201(1), 160-169. https://doi.org/10.1086/648589