Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513

Ai Yu Gong, Rui Zhou, Guoku Hu, Jun Liu, Danuta Sosnowska, Kristen M. Drescher, Haidong M Dong, Xian Ming Chen

Research output: Contribution to journalArticle

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Abstract

Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

Original languageEnglish (US)
Pages (from-to)160-169
Number of pages10
JournalJournal of Infectious Diseases
Volume201
Issue number1
DOIs
StatePublished - Jan 2010

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Cryptosporidium parvum
MicroRNAs
Parasites
Transfection
B7 Antigens
T-Lymphocytes
3' Untranslated Regions
Coculture Techniques
Infection
Neutralizing Antibodies
Cell Death
Epithelial Cells
Apoptosis
Proteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Gong, A. Y., Zhou, R., Hu, G., Liu, J., Sosnowska, D., Drescher, K. M., ... Chen, X. M. (2010). Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. Journal of Infectious Diseases, 201(1), 160-169. https://doi.org/10.1086/648589

Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. / Gong, Ai Yu; Zhou, Rui; Hu, Guoku; Liu, Jun; Sosnowska, Danuta; Drescher, Kristen M.; Dong, Haidong M; Chen, Xian Ming.

In: Journal of Infectious Diseases, Vol. 201, No. 1, 01.2010, p. 160-169.

Research output: Contribution to journalArticle

Gong, AY, Zhou, R, Hu, G, Liu, J, Sosnowska, D, Drescher, KM, Dong, HM & Chen, XM 2010, 'Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513', Journal of Infectious Diseases, vol. 201, no. 1, pp. 160-169. https://doi.org/10.1086/648589
Gong, Ai Yu ; Zhou, Rui ; Hu, Guoku ; Liu, Jun ; Sosnowska, Danuta ; Drescher, Kristen M. ; Dong, Haidong M ; Chen, Xian Ming. / Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. In: Journal of Infectious Diseases. 2010 ; Vol. 201, No. 1. pp. 160-169.
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