Correlations between vaccinia-specific immune responses within a cohort of armed forces members

Benjamin J. Umlauf, Inna G. Ovsyannikova, Iana H. Haralambieva, Richard B Kennedy, Robert A. Vierkant, V. Shane Pankratz, Robert M. Jacobson, Gregory A. Poland

Research output: Contribution to journalArticle

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Abstract

Widespread vaccination with vaccinia virus (VACV) resulted in the eradication of smallpox; however, the licensed VACV-containing vaccines are associated with adverse events (AEs), making them unsuitable for certain high-risk populations. A better understanding of the host immune response following smallpox vaccination could result in vaccines with similar immunogenicity profiles to pre-eradication vaccines with a lower incidence of AEs. To study the immune response to VACV, we recruited 1,076 armed forces members who had been vaccinated with one dose of Dryvax ®. We measured multiple VACV-specific immune responses: neutralizing antibody titer, the level of 12 secreted cytokines in peripheral blood mononuclear cell (PBMC) cultures (IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, TNF-α, IFN-γ, IFN-α, IFN-β, and IL-18), and the number of IFN-γ-and CD8 + IFN-γ-secreting cells. We analyzed these data to determine correlations between immune response measures. We detected a strong proinflammatory response in concert with a Th-1-like cytokine response pattern at a median time point of 15.3mo following primary vaccination. We also detected correlations between neutralizing antibody titer and secreted IL-2, as well as secreted IFN-γ (p=0.009 and p=0.0007, respectively). We also detected strong correlations between the proinflammatory cytokines IL-1β, TNF-α, IL-6, and IL-12p40 (p<0.0001). These results further advance our knowledge of vaccinia-specific cellular immune responses. Notably, vaccine-induced proinflammatory responses were not correlated with neutralizing antibody titers, suggesting that further attenuation to reduce inflammatory immune responses may result in decreased AEs without sacrificing VACV immunogenicity and population seropositivity.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalViral Immunology
Volume24
Issue number5
DOIs
StatePublished - Oct 1 2011

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Vaccinia
Vaccinia virus
Vaccines
Neutralizing Antibodies
Interleukin-12 Subunit p40
Vaccination
Smallpox
Cytokines
Interleukin-1
Interleukin-2
Interleukin-6
Smallpox Vaccine
Interleukin-18
Cellular Immunity
Interleukin-4
Interleukin-10
Population
Blood Cells
Cell Culture Techniques
Incidence

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Molecular Medicine

Cite this

Umlauf, B. J., Ovsyannikova, I. G., Haralambieva, I. H., Kennedy, R. B., Vierkant, R. A., Pankratz, V. S., ... Poland, G. A. (2011). Correlations between vaccinia-specific immune responses within a cohort of armed forces members. Viral Immunology, 24(5), 415-420. https://doi.org/10.1089/vim.2011.0029

Correlations between vaccinia-specific immune responses within a cohort of armed forces members. / Umlauf, Benjamin J.; Ovsyannikova, Inna G.; Haralambieva, Iana H.; Kennedy, Richard B; Vierkant, Robert A.; Pankratz, V. Shane; Jacobson, Robert M.; Poland, Gregory A.

In: Viral Immunology, Vol. 24, No. 5, 01.10.2011, p. 415-420.

Research output: Contribution to journalArticle

Umlauf, BJ, Ovsyannikova, IG, Haralambieva, IH, Kennedy, RB, Vierkant, RA, Pankratz, VS, Jacobson, RM & Poland, GA 2011, 'Correlations between vaccinia-specific immune responses within a cohort of armed forces members', Viral Immunology, vol. 24, no. 5, pp. 415-420. https://doi.org/10.1089/vim.2011.0029
Umlauf BJ, Ovsyannikova IG, Haralambieva IH, Kennedy RB, Vierkant RA, Pankratz VS et al. Correlations between vaccinia-specific immune responses within a cohort of armed forces members. Viral Immunology. 2011 Oct 1;24(5):415-420. https://doi.org/10.1089/vim.2011.0029
Umlauf, Benjamin J. ; Ovsyannikova, Inna G. ; Haralambieva, Iana H. ; Kennedy, Richard B ; Vierkant, Robert A. ; Pankratz, V. Shane ; Jacobson, Robert M. ; Poland, Gregory A. / Correlations between vaccinia-specific immune responses within a cohort of armed forces members. In: Viral Immunology. 2011 ; Vol. 24, No. 5. pp. 415-420.
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