Correlates of IL-10 and IL-12 concentrations in cervical secretions

Patti E. Gravitt, Allan Hildesheim, Rolando Herrero, Mark Schiffman, Mark E. Sherman, M. Concepcion Bratti, Ana C. Rodriguez, Lidia A. Morera, Fernando Cardenas, Frederick P. Bowman, Keerti V. Shah, Peggy A. Crowley-Nowick

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Interindividual variations in host immune responses to HPV infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer, However, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). We sought to determine biologic correlates of IL-10 and IL-12 concentrations in cervical secretions. Cervical secretions were passively collected using a WeckCel sponge from 247 women participating in a natural history study of human papillomavirus infection as part of an immunologic ancillary study. IL-10 and IL-12 concentrations were determined using standard ELISA assays. In general, IL-10 and IL-12 levels were significantly intercorrelated (Pearson's correlation coefficient = 0.6) but had somewhat different determinants. Significant increases (P < 0.05) in IL-10 concentrations were observed for nonovulatory phases of the menstrual cycle, postmenopausal status, recent use of oral contraceptives (OC), low secretion volume, macrolevels of heme contamination, and high vaginal pH. Increasing IL- 10 levels were also observed among smokers, women with increasing numbers of lifetime sex partners, and women who report having less frequent sex (less than once per week), however, these resuits were not statistically significant. Significantly higher IL-12 concentrations were observed among recent OC users, women with low secretion volume, and women with a high vaginal pH. There was a non-statistically significant observation of increasing IL-12 levels among nonsmokers, women with increasing numbers of lifetime and recent pregnancies, and increasing levels of heme contamination. We failed to observe a significant association between HPV and IL- 10 or IL- 12 levels in this cross-sectional sample. Future analyses of cervical cytokine levels and HPV infection should control for the inherent variation of local cytokine levels due to hormonal influences, hemoglobin contamination, pH, and cervical secretion volume differences.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalJournal of Clinical Immunology
Volume23
Issue number3
DOIs
StatePublished - May 2003

Fingerprint

Interleukin-12
Interleukin-10
Oral Contraceptives
Heme
Cytokines
Cervical Intraepithelial Neoplasia
Papillomavirus Infections
Porifera
Menstrual Cycle
Infection Control
Infection
Natural History
Mucous Membrane
Hemoglobins
Biomarkers
Enzyme-Linked Immunosorbent Assay
Observation
Pregnancy
Neoplasms

Keywords

  • Cervix
  • Mucosal
  • Secretions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Gravitt, P. E., Hildesheim, A., Herrero, R., Schiffman, M., Sherman, M. E., Bratti, M. C., ... Crowley-Nowick, P. A. (2003). Correlates of IL-10 and IL-12 concentrations in cervical secretions. Journal of Clinical Immunology, 23(3), 175-183. https://doi.org/10.1023/A:1023305827971

Correlates of IL-10 and IL-12 concentrations in cervical secretions. / Gravitt, Patti E.; Hildesheim, Allan; Herrero, Rolando; Schiffman, Mark; Sherman, Mark E.; Bratti, M. Concepcion; Rodriguez, Ana C.; Morera, Lidia A.; Cardenas, Fernando; Bowman, Frederick P.; Shah, Keerti V.; Crowley-Nowick, Peggy A.

In: Journal of Clinical Immunology, Vol. 23, No. 3, 05.2003, p. 175-183.

Research output: Contribution to journalArticle

Gravitt, PE, Hildesheim, A, Herrero, R, Schiffman, M, Sherman, ME, Bratti, MC, Rodriguez, AC, Morera, LA, Cardenas, F, Bowman, FP, Shah, KV & Crowley-Nowick, PA 2003, 'Correlates of IL-10 and IL-12 concentrations in cervical secretions', Journal of Clinical Immunology, vol. 23, no. 3, pp. 175-183. https://doi.org/10.1023/A:1023305827971
Gravitt, Patti E. ; Hildesheim, Allan ; Herrero, Rolando ; Schiffman, Mark ; Sherman, Mark E. ; Bratti, M. Concepcion ; Rodriguez, Ana C. ; Morera, Lidia A. ; Cardenas, Fernando ; Bowman, Frederick P. ; Shah, Keerti V. ; Crowley-Nowick, Peggy A. / Correlates of IL-10 and IL-12 concentrations in cervical secretions. In: Journal of Clinical Immunology. 2003 ; Vol. 23, No. 3. pp. 175-183.
@article{1d351561a3ec423d904eede9faba3951,
title = "Correlates of IL-10 and IL-12 concentrations in cervical secretions",
abstract = "Interindividual variations in host immune responses to HPV infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer, However, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). We sought to determine biologic correlates of IL-10 and IL-12 concentrations in cervical secretions. Cervical secretions were passively collected using a WeckCel sponge from 247 women participating in a natural history study of human papillomavirus infection as part of an immunologic ancillary study. IL-10 and IL-12 concentrations were determined using standard ELISA assays. In general, IL-10 and IL-12 levels were significantly intercorrelated (Pearson's correlation coefficient = 0.6) but had somewhat different determinants. Significant increases (P < 0.05) in IL-10 concentrations were observed for nonovulatory phases of the menstrual cycle, postmenopausal status, recent use of oral contraceptives (OC), low secretion volume, macrolevels of heme contamination, and high vaginal pH. Increasing IL- 10 levels were also observed among smokers, women with increasing numbers of lifetime sex partners, and women who report having less frequent sex (less than once per week), however, these resuits were not statistically significant. Significantly higher IL-12 concentrations were observed among recent OC users, women with low secretion volume, and women with a high vaginal pH. There was a non-statistically significant observation of increasing IL-12 levels among nonsmokers, women with increasing numbers of lifetime and recent pregnancies, and increasing levels of heme contamination. We failed to observe a significant association between HPV and IL- 10 or IL- 12 levels in this cross-sectional sample. Future analyses of cervical cytokine levels and HPV infection should control for the inherent variation of local cytokine levels due to hormonal influences, hemoglobin contamination, pH, and cervical secretion volume differences.",
keywords = "Cervix, Mucosal, Secretions",
author = "Gravitt, {Patti E.} and Allan Hildesheim and Rolando Herrero and Mark Schiffman and Sherman, {Mark E.} and Bratti, {M. Concepcion} and Rodriguez, {Ana C.} and Morera, {Lidia A.} and Fernando Cardenas and Bowman, {Frederick P.} and Shah, {Keerti V.} and Crowley-Nowick, {Peggy A.}",
year = "2003",
month = "5",
doi = "10.1023/A:1023305827971",
language = "English (US)",
volume = "23",
pages = "175--183",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Correlates of IL-10 and IL-12 concentrations in cervical secretions

AU - Gravitt, Patti E.

AU - Hildesheim, Allan

AU - Herrero, Rolando

AU - Schiffman, Mark

AU - Sherman, Mark E.

AU - Bratti, M. Concepcion

AU - Rodriguez, Ana C.

AU - Morera, Lidia A.

AU - Cardenas, Fernando

AU - Bowman, Frederick P.

AU - Shah, Keerti V.

AU - Crowley-Nowick, Peggy A.

PY - 2003/5

Y1 - 2003/5

N2 - Interindividual variations in host immune responses to HPV infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer, However, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). We sought to determine biologic correlates of IL-10 and IL-12 concentrations in cervical secretions. Cervical secretions were passively collected using a WeckCel sponge from 247 women participating in a natural history study of human papillomavirus infection as part of an immunologic ancillary study. IL-10 and IL-12 concentrations were determined using standard ELISA assays. In general, IL-10 and IL-12 levels were significantly intercorrelated (Pearson's correlation coefficient = 0.6) but had somewhat different determinants. Significant increases (P < 0.05) in IL-10 concentrations were observed for nonovulatory phases of the menstrual cycle, postmenopausal status, recent use of oral contraceptives (OC), low secretion volume, macrolevels of heme contamination, and high vaginal pH. Increasing IL- 10 levels were also observed among smokers, women with increasing numbers of lifetime sex partners, and women who report having less frequent sex (less than once per week), however, these resuits were not statistically significant. Significantly higher IL-12 concentrations were observed among recent OC users, women with low secretion volume, and women with a high vaginal pH. There was a non-statistically significant observation of increasing IL-12 levels among nonsmokers, women with increasing numbers of lifetime and recent pregnancies, and increasing levels of heme contamination. We failed to observe a significant association between HPV and IL- 10 or IL- 12 levels in this cross-sectional sample. Future analyses of cervical cytokine levels and HPV infection should control for the inherent variation of local cytokine levels due to hormonal influences, hemoglobin contamination, pH, and cervical secretion volume differences.

AB - Interindividual variations in host immune responses to HPV infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer, However, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). We sought to determine biologic correlates of IL-10 and IL-12 concentrations in cervical secretions. Cervical secretions were passively collected using a WeckCel sponge from 247 women participating in a natural history study of human papillomavirus infection as part of an immunologic ancillary study. IL-10 and IL-12 concentrations were determined using standard ELISA assays. In general, IL-10 and IL-12 levels were significantly intercorrelated (Pearson's correlation coefficient = 0.6) but had somewhat different determinants. Significant increases (P < 0.05) in IL-10 concentrations were observed for nonovulatory phases of the menstrual cycle, postmenopausal status, recent use of oral contraceptives (OC), low secretion volume, macrolevels of heme contamination, and high vaginal pH. Increasing IL- 10 levels were also observed among smokers, women with increasing numbers of lifetime sex partners, and women who report having less frequent sex (less than once per week), however, these resuits were not statistically significant. Significantly higher IL-12 concentrations were observed among recent OC users, women with low secretion volume, and women with a high vaginal pH. There was a non-statistically significant observation of increasing IL-12 levels among nonsmokers, women with increasing numbers of lifetime and recent pregnancies, and increasing levels of heme contamination. We failed to observe a significant association between HPV and IL- 10 or IL- 12 levels in this cross-sectional sample. Future analyses of cervical cytokine levels and HPV infection should control for the inherent variation of local cytokine levels due to hormonal influences, hemoglobin contamination, pH, and cervical secretion volume differences.

KW - Cervix

KW - Mucosal

KW - Secretions

UR - http://www.scopus.com/inward/record.url?scp=0037407406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037407406&partnerID=8YFLogxK

U2 - 10.1023/A:1023305827971

DO - 10.1023/A:1023305827971

M3 - Article

VL - 23

SP - 175

EP - 183

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 3

ER -