Controlled release of small molecules for cardiac differentiation of pluripotent stem cells

Christopher J. Tsao, Francesca Taraballi, Laura Pandolfi, Aaron J. Velasquez-Mao, Rodrigo Ruano, Ennio Tasciotti, Jeffrey G. Jacot

Research output: Contribution to journalArticle

Abstract

Induced pluripotent stem cells (iPSCs) have been shown to differentiate to functional cardiomyocytes (CM) with high efficiency through temporally controlled inhibition of the GSK3/Wnt signaling pathways. In this study, we investigated the ability of temporally controlled release of GSK3/Wnt small-molecule inhibitors to drive cardiac differentiation of iPSC without manual intervention. Porous silica particles were loaded with GSK3 inhibitor CHIR99021 or Wnt inhibitor IWP2, and the particles containing IWP2 were coated with 5 wt% poly(lactic-co-glycolic acid) 50:50 to delay release by ∼72 h. iPSCs reprogrammed through mRNA transfection were cultured with these particles up to 30 days. High-performance liquid chromatography suggests a burst release of CHIR99021 within the first 24 h and a delayed release of IWP2 after 72 h. Annexin V/propidium iodide staining did not show a significant effect on apoptosis or necrosis rates. Cultured cells upregulated both early (Nkx 2.5, Isl-1) and late (cTnT, MHC, Cx43) cardiac markers, assayed with a quantitative real-time polymerase chain reaction, and began spontaneous contraction at 3.0 ± 0.6 Hz at 15-21 days after the start of differentiation. CM had clear sarcomeric striations when stained for β-myosin heavy chain, and showed expression and punctate membrane localization of gap junction protein Connexin43. Calcium and voltage-sensitive imaging showed both action potential and calcium transients typical of immature CM. This study showed that the cardiac differentiation of pluripotent stem cells can be directed by porous silica vectors with temporally controlled release of small-molecule inhibitors. These results suggest methods for automating and eliminating variability in manual maintenance of inhibitor concentrations in the differentiation of pluripotent stem cells to CM.

Original languageEnglish (US)
Pages (from-to)1798-1807
Number of pages10
JournalTissue Engineering - Part A
Volume24
Issue number23-24
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

Fingerprint

Pluripotent Stem Cells
Stem cells
Cardiac Myocytes
Induced Pluripotent Stem Cells
Connexin 43
Molecules
Silicon Dioxide
Calcium
Wnt Signaling Pathway
Connexins
Silica
Myosin Heavy Chains
Propidium
Annexin A5
Action Potentials
Polymerase chain reaction
Transfection
High performance liquid chromatography
Real-Time Polymerase Chain Reaction
Cell death

Keywords

  • Cardiac differentiation
  • Controlled release
  • IPS cells

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Tsao, C. J., Taraballi, F., Pandolfi, L., Velasquez-Mao, A. J., Ruano, R., Tasciotti, E., & Jacot, J. G. (2018). Controlled release of small molecules for cardiac differentiation of pluripotent stem cells. Tissue Engineering - Part A, 24(23-24), 1798-1807. https://doi.org/10.1089/ten.tea.2018.0054

Controlled release of small molecules for cardiac differentiation of pluripotent stem cells. / Tsao, Christopher J.; Taraballi, Francesca; Pandolfi, Laura; Velasquez-Mao, Aaron J.; Ruano, Rodrigo; Tasciotti, Ennio; Jacot, Jeffrey G.

In: Tissue Engineering - Part A, Vol. 24, No. 23-24, 01.12.2018, p. 1798-1807.

Research output: Contribution to journalArticle

Tsao, CJ, Taraballi, F, Pandolfi, L, Velasquez-Mao, AJ, Ruano, R, Tasciotti, E & Jacot, JG 2018, 'Controlled release of small molecules for cardiac differentiation of pluripotent stem cells', Tissue Engineering - Part A, vol. 24, no. 23-24, pp. 1798-1807. https://doi.org/10.1089/ten.tea.2018.0054
Tsao, Christopher J. ; Taraballi, Francesca ; Pandolfi, Laura ; Velasquez-Mao, Aaron J. ; Ruano, Rodrigo ; Tasciotti, Ennio ; Jacot, Jeffrey G. / Controlled release of small molecules for cardiac differentiation of pluripotent stem cells. In: Tissue Engineering - Part A. 2018 ; Vol. 24, No. 23-24. pp. 1798-1807.
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