TY - JOUR
T1 - Comparison of porcine and semisynthetic human insulins using euglycemic clamp-derived glucose-insulin dose-response curves in insulin-dependent diabetes
AU - Staten, Myrlene
AU - Worcester, Beverly
AU - Szekeres, Agnes
AU - Waldeck, Nancy
AU - Ascher, Michael
AU - Walsh, Kathleen M.
AU - Rizza, Robert
AU - Gerich, John
AU - Charles, M. Arthur
N1 - Funding Information:
From the Diabetes Research Program and Departments of Medicine and Physiology, University of California, Irvine, California. Novo Industries, Wilton, Connecticut, and the Endocrine Research Unit, Mayo Medical School and Mayo Clinic, Rochester. Minnesota. Received for publication April 19, 1983. This study was supported by Novo Industries and the UC1 Focused Research Program in Diabetes. Address reprint requests to Dr M. A. Charles. Rm C-240, Med Sci I. Department of Medicine, University of California, Irvine, CA 92717. o 1984 by Grune & Stratton, Inc. 00.?6-a495/84/3302-0008$01.00/0
PY - 1984/2
Y1 - 1984/2
N2 - In order to compare the biologic effectiveness of porcine and semisynthetic human insulins, a euglycemic clamp method was used in eight insulin-dependent diabetic subjects. Each subject was tested for each insulin on separate days. In order to derive glucose-insulin dose-response curves for both insulins, sequential but constant insulin infusion rates of 0.2, 0.5, 1.0, and 2.0 mU/kg/min were performed. Plasma glucose levels attained during the euglycemic clamp were 96 ± 3 mg/dL. At each insulin infusion rate, the steady-state glucose infusion rate required to maintain euglycemia was measured. At each increment of insulin infused, steady-state glucose infusion rates for porcine insulin were 1.12 ± 0.22, 1.90 ± 0.59, 4.28 ± 0.61, and 9.37 ± 0.66 mg/kg/min compared with 1.27 ± 0.42, 2.38 ± 0.20, 4.25 ± 0.43, and 8.87 ± 0.67 mg/kg/min for semisynthetic human insulin. By ANOVA, no significant difference was noted between the two insulins. Because insulin infusion rates may not result in predictable circulating free insulin levels in subjects who have circulating insulin antibodies, free insulin levels were determined. When steady-state glucose infusion rates were compared with free insulin levels achieved at the four insulin infusion rates, dose-response curves for both porcine and semisynthetic human insulins were virtually identical. These data suggest that semisynthetic human insulin has equivalent biologic effects on overall glucose metabolism compared with porcine insulin in insulin-dependent diabetes.
AB - In order to compare the biologic effectiveness of porcine and semisynthetic human insulins, a euglycemic clamp method was used in eight insulin-dependent diabetic subjects. Each subject was tested for each insulin on separate days. In order to derive glucose-insulin dose-response curves for both insulins, sequential but constant insulin infusion rates of 0.2, 0.5, 1.0, and 2.0 mU/kg/min were performed. Plasma glucose levels attained during the euglycemic clamp were 96 ± 3 mg/dL. At each insulin infusion rate, the steady-state glucose infusion rate required to maintain euglycemia was measured. At each increment of insulin infused, steady-state glucose infusion rates for porcine insulin were 1.12 ± 0.22, 1.90 ± 0.59, 4.28 ± 0.61, and 9.37 ± 0.66 mg/kg/min compared with 1.27 ± 0.42, 2.38 ± 0.20, 4.25 ± 0.43, and 8.87 ± 0.67 mg/kg/min for semisynthetic human insulin. By ANOVA, no significant difference was noted between the two insulins. Because insulin infusion rates may not result in predictable circulating free insulin levels in subjects who have circulating insulin antibodies, free insulin levels were determined. When steady-state glucose infusion rates were compared with free insulin levels achieved at the four insulin infusion rates, dose-response curves for both porcine and semisynthetic human insulins were virtually identical. These data suggest that semisynthetic human insulin has equivalent biologic effects on overall glucose metabolism compared with porcine insulin in insulin-dependent diabetes.
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U2 - 10.1016/0026-0495(84)90125-2
DO - 10.1016/0026-0495(84)90125-2
M3 - Article
C2 - 6363874
AN - SCOPUS:0021329261
SN - 0026-0495
VL - 33
SP - 132
EP - 135
JO - Metabolism
JF - Metabolism
IS - 2
ER -