Comparison of porcine and semisynthetic human insulins using euglycemic clamp-derived glucose-insulin dose-response curves in insulin-dependent diabetes

Myrlene Staten, Beverly Worcester, Agnes Szekeres, Nancy Waldeck, Michael Ascher, Kathleen M. Walsh, Robert Rizza, John Gerich, M. Arthur Charles

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In order to compare the biologic effectiveness of porcine and semisynthetic human insulins, a euglycemic clamp method was used in eight insulin-dependent diabetic subjects. Each subject was tested for each insulin on separate days. In order to derive glucose-insulin dose-response curves for both insulins, sequential but constant insulin infusion rates of 0.2, 0.5, 1.0, and 2.0 mU/kg/min were performed. Plasma glucose levels attained during the euglycemic clamp were 96 ± 3 mg/dL. At each insulin infusion rate, the steady-state glucose infusion rate required to maintain euglycemia was measured. At each increment of insulin infused, steady-state glucose infusion rates for porcine insulin were 1.12 ± 0.22, 1.90 ± 0.59, 4.28 ± 0.61, and 9.37 ± 0.66 mg/kg/min compared with 1.27 ± 0.42, 2.38 ± 0.20, 4.25 ± 0.43, and 8.87 ± 0.67 mg/kg/min for semisynthetic human insulin. By ANOVA, no significant difference was noted between the two insulins. Because insulin infusion rates may not result in predictable circulating free insulin levels in subjects who have circulating insulin antibodies, free insulin levels were determined. When steady-state glucose infusion rates were compared with free insulin levels achieved at the four insulin infusion rates, dose-response curves for both porcine and semisynthetic human insulins were virtually identical. These data suggest that semisynthetic human insulin has equivalent biologic effects on overall glucose metabolism compared with porcine insulin in insulin-dependent diabetes.

Original languageEnglish (US)
Pages (from-to)132-135
Number of pages4
JournalMetabolism
Volume33
Issue number2
DOIs
StatePublished - Feb 1984

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Staten, M., Worcester, B., Szekeres, A., Waldeck, N., Ascher, M., Walsh, K. M., Rizza, R., Gerich, J., & Charles, M. A. (1984). Comparison of porcine and semisynthetic human insulins using euglycemic clamp-derived glucose-insulin dose-response curves in insulin-dependent diabetes. Metabolism, 33(2), 132-135. https://doi.org/10.1016/0026-0495(84)90125-2