Comparison of nerve regeneration in vascularized and conventional grafts: nerve electrophysiology, norepinephrine, prostacyclin, malondialdehyde, and the blood-nerve barrier

M. Shupeck, K. K. Ward, J. D. Schmelzer, Phillip Anson Low

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We compared the efficacy of vascularized (VASC) and conventional (CONV) sciatic nerve grafts in restoring nerve blood flow (NBF). conduction, the blood-nerve barrier, norepinephrine (NE), and 6-keto prostaglandin F (6-KPGF; the stable prostacyclin metabolite) in the sciatic nerve of the rat. We also measured malondialdehyde (MDA) content. NBF was much greater in VASC grafts, but the increase was confined to non-nutritive flow. There was a statistically non-significant increase in nerve action potential amplitude in the grafted segments of VASC nerves at 1 and 2 months post graft. The [14C]sucrose permeability surface area (PA) product was increased in both CONV and VASC at 1 and 3 months and was not different to each other. NE and 6-KPGF, the major vasoconstrictor and dilator of nerve microvessels were better restored in VASC than CONV reaching statistically significance for 6-KPGF (P < 0.001). MDA used as an index of oxygen free radical generation was not significantly different in the 3 groups. The better restoration of 6-KPGF and perhaps NE suggest that VASC grafts may be more effective in restoring vasoreactivity of peripheral nerve following graft.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalBrain Research
Volume493
Issue number2
DOIs
StatePublished - Jul 31 1989

Fingerprint

Blood-Nerve Barrier
Nerve Regeneration
Electrophysiology
Epoprostenol
Malondialdehyde
Norepinephrine
Transplants
Sciatic Nerve
Vasoconstrictor Agents
Microvessels
Peripheral Nerves
Action Potentials
Free Radicals
Sucrose
Permeability
Reactive Oxygen Species

Keywords

  • Blood-nerve barrier
  • Electrophysiology
  • Malondialdehyde
  • Nerve graft
  • Noradrenaline
  • Prostacyclin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Comparison of nerve regeneration in vascularized and conventional grafts : nerve electrophysiology, norepinephrine, prostacyclin, malondialdehyde, and the blood-nerve barrier. / Shupeck, M.; Ward, K. K.; Schmelzer, J. D.; Low, Phillip Anson.

In: Brain Research, Vol. 493, No. 2, 31.07.1989, p. 225-230.

Research output: Contribution to journalArticle

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