Comparison of line probe assay and DNA sequencing of 5′ untranslated region for genotyping hepatitis C virus: Description of novel line probe patterns

P. Shawn Mitchell, Lynne M. Sloan, David W. Majewski, Paul N. Rys, Paul J. Heimgartner, Jon E. Rosenblatt, Franklin R. Cockerill, Thomas F. Smith, Robin Patel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We compared a commercial line probe assay (INNO-LiPA™ HCV II, Innogenetics, N.V., Ghent, Belgium, distributed by Bayer Diagnostics) to an in-house 5′ untranslated region direct DNA sequencing method for genotyping hepatitis C virus (HCV). Initial evaluation demonstrated that the INNO-LiPA™ HCV II assay and sequencing assay assigned the same genotype for 110/132 (83.3%) patient specimens (98 subtype and 12 genotype only identifications). Following the initial evaluation, the INNO-LiPA™ HCV II assay was used routinely to genotype HCV from patient specimens submitted to our laboratory for genotyping (n = 1,739). During this second part of the study, novel line probe patterns have been noted and interpreted using the in-house direct sequencing assay. Reactivity at bands 1, 2, 3, 4, 5 and 8 (n = 4) or 1, 2, 3, 4, 6 and 7 (n = 2) represented HCV genotype 1. Reactivity at bands 1, 2, 5 and 9 (n = 1) represented HCV genotype 2. Reactivity at bands 1, 2, 5, 9 and 16 (n = 1) represented HCV genotype 4. Reactivity at bands 1, 2, 5, 9, 10, 11 (weak band) and 12 (n = 118) most likely represented HCV genotype 2b. This information should be of use to INNO-LiPA™ HCV II assay users.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume42
Issue number3
DOIs
StatePublished - Apr 9 2002

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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