Comparison of human papillomavirus testing and cytology for cervical cancer screening in a primary health care setting in the Democratic Republic of the Congo

Salaheddin M. Mahmud, Ghislain Sangwa-Lugoma, Samih H. Nasr, Patrick K. Kayembe, Rahma R. Tozin, Pierre Drouin, Attila Lorincz, Alex Ferenczy, Eduardo L. Franco

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Objectives: We compared the screening performance of conventional Pap cytology and two human papillomavirus (HPV) DNA assays, the original Hybrid Capture 2 (HC2) and an expanded version that tests for 4 additional HPV types (HC2 + 4; Qiagen Corporation), in the detection of cervical neoplasia among unscreened women in a primary care setting in a suburb of Kinshasa, Democratic Republic of the Congo. Methods: All women 30 years or older residing in the area were invited to participate, and 1528 were evaluated by Pap cytology and the two HPV assays, conducted at a European and US reference laboratory, respectively, followed by colposcopy. Cervical biopsies were obtained from all women with abnormal colposcopy and from 290 randomly chosen women with normal colposcopy (to correct for verification bias). Results: Using a relative light unit of 1 as the cutoff for positivity, 169 and 168 (11%) women tested positive using HC2 and HC2 + 4, respectively. HC2 and HC2 + 4 were in agreement in 98.6% of cases (Kappa = 0.94; 95% confidence interval: 0.91-0.96). Both assays were sensitive (∼ 83%) and specific (∼ 91%) for the detection of cervical intraepithelial neoplasia-2 or worse disease. Irrespective of the cutoff point used to define positivity, Pap cytology was both less sensitive and more specific than HC2 or HC2 + 4. For instance, cytology was 63% sensitive and 97% specific when a cutoff point of low-grade squamous intraepithelial lesions or worse was used. Conclusions: Among unscreened women, HC2 and HC2 + 4 had similar screening accuracy for cervical neoplasia, and both were more sensitive but less specific than Pap cytology.

Original languageEnglish (US)
Pages (from-to)286-291
Number of pages6
JournalGynecologic oncology
Volume124
Issue number2
DOIs
StatePublished - Feb 2012

Keywords

  • Cervical cancer
  • Cervical intraepithelial neoplasia
  • Human papillomavirus
  • Pap cytology
  • Screening

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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    Mahmud, S. M., Sangwa-Lugoma, G., Nasr, S. H., Kayembe, P. K., Tozin, R. R., Drouin, P., Lorincz, A., Ferenczy, A., & Franco, E. L. (2012). Comparison of human papillomavirus testing and cytology for cervical cancer screening in a primary health care setting in the Democratic Republic of the Congo. Gynecologic oncology, 124(2), 286-291. https://doi.org/10.1016/j.ygyno.2011.10.031