Comparison of GLUT1, GLUT3, and GLUT4 mRNA and the subcellular distribution of their proteins in normal human muscle

Charles A. Stuart, Gary Wen, W. Clay Gustafson, E Aubrey Thompson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Basal, "insulin-independent" glucose uptake into skeletal muscle is provided by glucose transporters positioned at the plasma membrane. The relative amount of the three glucose transporters expressed in muscle has not been previously quantified. Using a combination of qualitative and quantitative ribonuclease protection assay (RPA) methods, we found in normal human muscle that GLUT1, GLUT3, and GLUT4 mRNA were expressed at 90 ± 10, 46 ± 4, and 156 ± 12 copies/ng RNA, respectively. Muscle was fractionated by DNase digestion and differential sedimentation into membrane fractions enriched in plasma membranes (PM) or low-density microsomes (LDM). GLUT1 and GLUT4 proteins were distributed 57% to 67% in LDM, whereas GLUT3 protein was at least 88% in the PM-enriched fractions. These data suggest that basal glucose uptake into resting human muscle could be provided in part by each of these three isoforms.

Original languageEnglish (US)
Pages (from-to)1604-1609
Number of pages6
JournalMetabolism: Clinical and Experimental
Volume49
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

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Muscles
Messenger RNA
Facilitative Glucose Transport Proteins
Cell Membrane
Microsomes
Glucose Transporter Type 3
Proteins
Glucose Transporter Type 1
Glucose Transporter Type 4
Glucose
Deoxyribonucleases
Ribonucleases
Digestion
Protein Isoforms
Skeletal Muscle
RNA
Insulin
Membranes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Comparison of GLUT1, GLUT3, and GLUT4 mRNA and the subcellular distribution of their proteins in normal human muscle. / Stuart, Charles A.; Wen, Gary; Gustafson, W. Clay; Thompson, E Aubrey.

In: Metabolism: Clinical and Experimental, Vol. 49, No. 12, 2000, p. 1604-1609.

Research output: Contribution to journalArticle

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