Comparison of extracellular and net glucose oxidation measured isotopically and by indirect calorimetry during high and low glucose turnover

M. Molly McMahon, H. Michael Marsh, Robert A. Rizza

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To determine the extent to which glucose oxidation measured by indirect calorimetry reflects glucose oxidation measured isotopically, subjects were studied during a 6-h hyperinsulinemic euglycemic clamp (1 mU · kg-1 · min-1) and during infusion of saline. [6-14C]glucose was infused on both occasions. Breath was collected for determination of the specific activity of carbon dioxide, oxygen consumption, and carbon dioxide production. Glucose turnover during hyperinsulinemia was approximately eightfold higher than during saline infusion. During the final 1.5 h of the hyperinsulinemic glucose clamp, oxidation measured isotopically remained slightly but consistently lower (P < 0.05) than that measured by indirect calorimetry(13.8 ± 1.1 vs 16.5 ± 1.7 μmol · kg-1 · min-1, respectively). In contrast, during the saline infusion, glucose oxidation measured isotopically did not differ from that measured by indirect calorimetry (8.3 ± 0.6 vs 7.2 ± 2.8 μmol · kg-1 · min-1, respectively). We conclude that although net glucose oxidation measured isotopically was slightly lower than that measured by indirect calorimetry, both techniques provide similar estimates of glucose oxidation over a wide range of glucose disposal.

Original languageEnglish (US)
Pages (from-to)1138-1142
Number of pages5
JournalAmerican Journal of Clinical Nutrition
Volume53
Issue number5
StatePublished - May 1991

Fingerprint

Indirect Calorimetry
calorimetry
oxidation
Glucose
glucose
Glucose Clamp Technique
Carbon Dioxide
Hyperinsulinism
hyperinsulinemia
Oxygen Consumption
oxygen consumption
carbon dioxide

Keywords

  • [6-c]glucose
  • Glucose oxidation
  • Hyperinsulinemic clamp
  • Indirect calorimetry

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Comparison of extracellular and net glucose oxidation measured isotopically and by indirect calorimetry during high and low glucose turnover. / McMahon, M. Molly; Marsh, H. Michael; Rizza, Robert A.

In: American Journal of Clinical Nutrition, Vol. 53, No. 5, 05.1991, p. 1138-1142.

Research output: Contribution to journalArticle

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