Comparison of estrogen receptors in human premenopausal and postmenopausal uteri: Indication of biologically inactive receptor in postmenopausal uteri

J. H. Strathy, C. B. Coulam, T. C. Spelsberg

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Analysis of the estrogen receptor (ER) from uteri of premenopausal and postmenopausal patients has largely been limited to quantitation. The present study examined the biologic activity of the receptors with regard to their nuclear binding capacities. A Scatchard analysis of myometrial cytosol indicated high-affinity and low-capacity estradiol binding (K(d) = 10-10M). A 35% ammonium sulfate fraction of cytosol was characterized for steroid specificity, receptor concentration, isoelectric focusing patterns, and capacity of the ER to bind to nuclear acceptor sites in a cell-free assay. The ER from premenopausal uteri displayed an extensive but saturable binding to the nuclear acceptor sites. In contrast, the postmenopausal ER displayed minimal binding. These cell-free binding differences were supported by tissue culture studies. The difference may be accentuated by the fact that much of the receptor is bound by endogenous estrogen in the premenopausal uterine preparation, which would decrease the subsequent binding of the [3H]ER to nuclear sites. This receptor in the postmenopausal uterus is largely unbound and thus would not compete for nuclear sites. These studies suggest that a significant fraction of the ER in postmenopausal uteri is biologically inactive; for example, it cannot translocate and bind to nuclear acceptor sites. This loss in nuclear binding may represent a change in the ER occurring at menopause as a result of changing endocrine states.

Original languageEnglish (US)
Pages (from-to)372-382
Number of pages11
JournalAmerican journal of obstetrics and gynecology
Volume142
Issue number4
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Comparison of estrogen receptors in human premenopausal and postmenopausal uteri: Indication of biologically inactive receptor in postmenopausal uteri'. Together they form a unique fingerprint.

  • Cite this