Comparison of conventional ELISA with electrochemiluminescence technology for detection of amyloid-β in plasma

Esther S. Oh, Michelle M. Mielke, Paul B. Rosenberg, Alka Jain, Neal S. Fedarko, Constantine G. Lyketsos, Pankaj D. Mehta

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for th s may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.

Original languageEnglish (US)
Pages (from-to)769-773
Number of pages5
JournalJournal of Alzheimer's Disease
Volume21
Issue number3
DOIs
StatePublished - 2010

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Keywords

  • Amyloid-β protein
  • biological markers
  • enzyme-linked immunosorbent assay
  • plasma

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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