Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer

Robert E. Reiter, Issei Sato, George Thomas, Junqi Qian, Zhennan Gu, Tetsuro Watabe, Massimo Loda, Robert Brian Jenkins

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Gain of sequences on chromosome arm 8q is a common feature of prostate cancer that may correlate with metastatic and androgen-independent progression. The target gene(s) for this gain is not known, although MYC is amplified in a subset of advanced tumors and is one potential candidate. Prostate stem cell antigen (PSCA) is a prostate-specific cell surface protein that maps to chromosome region 8q24.2 and is overexpressed in prostate cancer. Our aim in this study was to test the hypothesis that PSCA overexpression may result from overrepresentation of chromosome arm 8q. Twenty locally advanced prostate cancers were analyzed by dual-probe fluorescence in situ hybridization (FISH) for alterations of MYC and PSCA. Extra copies of MYC were found in 12/20 (60%) tumors, including 5 (25%) with simple gain (no increase in MYC copy number relative to the chromosome 8 centromere) and 7 (35%) with an additional increase (AI or overrepresentation) in MYC copy number relative to the centromere. In the five cases with simple gain of MYC, there was a concomitant gain of PSCA. PSCA was overrepresented in 5/7 (71%) cases with AI of MYC. Immunohistochemical staining of the 20 tumors with monoclonal antibodies specific for PSCA showed a high degree of correlation between PSCA gene overrepresentation and protein overexpression. Four of 5 tumors with AI of PSCA overexpressed PSCA protein, compared with only 2/15 tumors with a normal PSCA copy number or simple gain of PSCA (P = 0.014). These results demonstrate that PSCA is co-overrepresented with MYC in a majority of cases, but may not be a necessary part of the 8q amplicon. PSCA protein overexpression can result from AI of PSCA and might be useful as a cell surface marker on prostate cancer cells with 8q overrepresentation.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalGenes Chromosomes and Cancer
Volume27
Issue number1
DOIs
StatePublished - Jan 2000

Fingerprint

Prostate
Prostatic Neoplasms
Stem Cells
Antigens
Centromere
Chromosomes
Neoplasms
Chromosomes, Human, Pair 8
Proteins
Chromosomes, Human, Pair 7
Fluorescence In Situ Hybridization
Androgens
Membrane Proteins
Monoclonal Antibodies
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer. / Reiter, Robert E.; Sato, Issei; Thomas, George; Qian, Junqi; Gu, Zhennan; Watabe, Tetsuro; Loda, Massimo; Jenkins, Robert Brian.

In: Genes Chromosomes and Cancer, Vol. 27, No. 1, 01.2000, p. 95-103.

Research output: Contribution to journalArticle

Reiter, Robert E. ; Sato, Issei ; Thomas, George ; Qian, Junqi ; Gu, Zhennan ; Watabe, Tetsuro ; Loda, Massimo ; Jenkins, Robert Brian. / Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer. In: Genes Chromosomes and Cancer. 2000 ; Vol. 27, No. 1. pp. 95-103.
@article{0238a5e4086a4b59a0158632293f1725,
title = "Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer",
abstract = "Gain of sequences on chromosome arm 8q is a common feature of prostate cancer that may correlate with metastatic and androgen-independent progression. The target gene(s) for this gain is not known, although MYC is amplified in a subset of advanced tumors and is one potential candidate. Prostate stem cell antigen (PSCA) is a prostate-specific cell surface protein that maps to chromosome region 8q24.2 and is overexpressed in prostate cancer. Our aim in this study was to test the hypothesis that PSCA overexpression may result from overrepresentation of chromosome arm 8q. Twenty locally advanced prostate cancers were analyzed by dual-probe fluorescence in situ hybridization (FISH) for alterations of MYC and PSCA. Extra copies of MYC were found in 12/20 (60{\%}) tumors, including 5 (25{\%}) with simple gain (no increase in MYC copy number relative to the chromosome 8 centromere) and 7 (35{\%}) with an additional increase (AI or overrepresentation) in MYC copy number relative to the centromere. In the five cases with simple gain of MYC, there was a concomitant gain of PSCA. PSCA was overrepresented in 5/7 (71{\%}) cases with AI of MYC. Immunohistochemical staining of the 20 tumors with monoclonal antibodies specific for PSCA showed a high degree of correlation between PSCA gene overrepresentation and protein overexpression. Four of 5 tumors with AI of PSCA overexpressed PSCA protein, compared with only 2/15 tumors with a normal PSCA copy number or simple gain of PSCA (P = 0.014). These results demonstrate that PSCA is co-overrepresented with MYC in a majority of cases, but may not be a necessary part of the 8q amplicon. PSCA protein overexpression can result from AI of PSCA and might be useful as a cell surface marker on prostate cancer cells with 8q overrepresentation.",
author = "Reiter, {Robert E.} and Issei Sato and George Thomas and Junqi Qian and Zhennan Gu and Tetsuro Watabe and Massimo Loda and Jenkins, {Robert Brian}",
year = "2000",
month = "1",
doi = "10.1002/(SICI)1098-2264(200001)27:1<95::AID-GCC12>3.0.CO;2-3",
language = "English (US)",
volume = "27",
pages = "95--103",
journal = "Genes Chromosomes and Cancer",
issn = "1045-2257",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced prostate cancer

AU - Reiter, Robert E.

AU - Sato, Issei

AU - Thomas, George

AU - Qian, Junqi

AU - Gu, Zhennan

AU - Watabe, Tetsuro

AU - Loda, Massimo

AU - Jenkins, Robert Brian

PY - 2000/1

Y1 - 2000/1

N2 - Gain of sequences on chromosome arm 8q is a common feature of prostate cancer that may correlate with metastatic and androgen-independent progression. The target gene(s) for this gain is not known, although MYC is amplified in a subset of advanced tumors and is one potential candidate. Prostate stem cell antigen (PSCA) is a prostate-specific cell surface protein that maps to chromosome region 8q24.2 and is overexpressed in prostate cancer. Our aim in this study was to test the hypothesis that PSCA overexpression may result from overrepresentation of chromosome arm 8q. Twenty locally advanced prostate cancers were analyzed by dual-probe fluorescence in situ hybridization (FISH) for alterations of MYC and PSCA. Extra copies of MYC were found in 12/20 (60%) tumors, including 5 (25%) with simple gain (no increase in MYC copy number relative to the chromosome 8 centromere) and 7 (35%) with an additional increase (AI or overrepresentation) in MYC copy number relative to the centromere. In the five cases with simple gain of MYC, there was a concomitant gain of PSCA. PSCA was overrepresented in 5/7 (71%) cases with AI of MYC. Immunohistochemical staining of the 20 tumors with monoclonal antibodies specific for PSCA showed a high degree of correlation between PSCA gene overrepresentation and protein overexpression. Four of 5 tumors with AI of PSCA overexpressed PSCA protein, compared with only 2/15 tumors with a normal PSCA copy number or simple gain of PSCA (P = 0.014). These results demonstrate that PSCA is co-overrepresented with MYC in a majority of cases, but may not be a necessary part of the 8q amplicon. PSCA protein overexpression can result from AI of PSCA and might be useful as a cell surface marker on prostate cancer cells with 8q overrepresentation.

AB - Gain of sequences on chromosome arm 8q is a common feature of prostate cancer that may correlate with metastatic and androgen-independent progression. The target gene(s) for this gain is not known, although MYC is amplified in a subset of advanced tumors and is one potential candidate. Prostate stem cell antigen (PSCA) is a prostate-specific cell surface protein that maps to chromosome region 8q24.2 and is overexpressed in prostate cancer. Our aim in this study was to test the hypothesis that PSCA overexpression may result from overrepresentation of chromosome arm 8q. Twenty locally advanced prostate cancers were analyzed by dual-probe fluorescence in situ hybridization (FISH) for alterations of MYC and PSCA. Extra copies of MYC were found in 12/20 (60%) tumors, including 5 (25%) with simple gain (no increase in MYC copy number relative to the chromosome 8 centromere) and 7 (35%) with an additional increase (AI or overrepresentation) in MYC copy number relative to the centromere. In the five cases with simple gain of MYC, there was a concomitant gain of PSCA. PSCA was overrepresented in 5/7 (71%) cases with AI of MYC. Immunohistochemical staining of the 20 tumors with monoclonal antibodies specific for PSCA showed a high degree of correlation between PSCA gene overrepresentation and protein overexpression. Four of 5 tumors with AI of PSCA overexpressed PSCA protein, compared with only 2/15 tumors with a normal PSCA copy number or simple gain of PSCA (P = 0.014). These results demonstrate that PSCA is co-overrepresented with MYC in a majority of cases, but may not be a necessary part of the 8q amplicon. PSCA protein overexpression can result from AI of PSCA and might be useful as a cell surface marker on prostate cancer cells with 8q overrepresentation.

UR - http://www.scopus.com/inward/record.url?scp=0033991344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033991344&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1098-2264(200001)27:1<95::AID-GCC12>3.0.CO;2-3

DO - 10.1002/(SICI)1098-2264(200001)27:1<95::AID-GCC12>3.0.CO;2-3

M3 - Article

VL - 27

SP - 95

EP - 103

JO - Genes Chromosomes and Cancer

JF - Genes Chromosomes and Cancer

SN - 1045-2257

IS - 1

ER -