Clots retrieved by mechanical thrombectomy from acute ischemic stroke patients show no evidence of bacteria

Zenith Khashim, Seán Fitzgerald, Ramanathan D Kadirvel, Daying Dai, Karen M. Doyle, Waleed Brinjikji, David F Kallmes

Research output: Contribution to journalArticle

Abstract

Background: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). Materials and methods: Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. Results: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. Conclusion: Our current study found no bacteria populations in the clots of AIS patients.

Original languageEnglish (US)
JournalInterventional Neuroradiology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Thrombectomy
Stroke
Bacteria
Ribosomal DNA
Gram-Negative Bacteria
Real-Time Polymerase Chain Reaction
Staining and Labeling
Blood Coagulation Factors
Population
Thrombosis
Demography

Keywords

  • acute ischemic stroke
  • Bacteria
  • mechanical thrombectomy

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Clots retrieved by mechanical thrombectomy from acute ischemic stroke patients show no evidence of bacteria. / Khashim, Zenith; Fitzgerald, Seán; Kadirvel, Ramanathan D; Dai, Daying; Doyle, Karen M.; Brinjikji, Waleed; Kallmes, David F.

In: Interventional Neuroradiology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Background: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). Materials and methods: Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. Results: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. Conclusion: Our current study found no bacteria populations in the clots of AIS patients.",
keywords = "acute ischemic stroke, Bacteria, mechanical thrombectomy",
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AU - Khashim, Zenith

AU - Fitzgerald, Seán

AU - Kadirvel, Ramanathan D

AU - Dai, Daying

AU - Doyle, Karen M.

AU - Brinjikji, Waleed

AU - Kallmes, David F

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N2 - Background: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). Materials and methods: Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. Results: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. Conclusion: Our current study found no bacteria populations in the clots of AIS patients.

AB - Background: Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS). Materials and methods: Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria. Results: Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples. Conclusion: Our current study found no bacteria populations in the clots of AIS patients.

KW - acute ischemic stroke

KW - Bacteria

KW - mechanical thrombectomy

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