Cloning of the human twist gene: Its expression is retained in adult mesodermally-derived tissues

Sherry M. Wang, Vincent W. Coljee, Robert J. Pignolo, Mitch O. Rotenberg, Vincent J. Cristofalo, Felipe Sierra

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalGene
Volume187
Issue number1
DOIs
StatePublished - Mar 10 1997

Keywords

  • Aging
  • Genomic DNA
  • Helix-loop-helix
  • Tissue-specific expression
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

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