Cloning of the human twist gene: Its expression is retained in adult mesodermally-derived tissues

Sherry M. Wang; Vincent W. Coljee; Robert J. Pignolo; Mitch O. Rotenberg; Vincent J. Cristofalo; Felipe Sierra

doi:10.1016/S0378-1119(96)00727-5

Cloning of the human twist gene: Its expression is retained in adult mesodermally-derived tissues

Sherry M. Wang, Vincent W. Coljee, Robert J. Pignolo, Mitch O. Rotenberg, Vincent J. Cristofalo, Felipe Sierra

Hospital Internal Medicine

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.

Original language	English (US)
Pages (from-to)	83-92
Number of pages	10
Journal	Gene
Volume	187
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(96)00727-5
State	Published - Mar 10 1997

Keywords

Aging
Genomic DNA
Helix-loop-helix
Tissue-specific expression
Transcription factor

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(96)00727-5

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title = "Cloning of the human twist gene: Its expression is retained in adult mesodermally-derived tissues",

abstract = "We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.",

keywords = "Aging, Genomic DNA, Helix-loop-helix, Tissue-specific expression, Transcription factor",

author = "Wang, {Sherry M.} and Coljee, {Vincent W.} and Pignolo, {Robert J.} and Rotenberg, {Mitch O.} and Cristofalo, {Vincent J.} and Felipe Sierra",

note = "Funding Information: We wish to thank Dr. Marie A. DiBerardino for her helpful discussion and comments. This work was supported by NIH grant AG 00378 to V.J.C.",

year = "1997",

month = mar,

day = "10",

doi = "10.1016/S0378-1119(96)00727-5",

language = "English (US)",

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pages = "83--92",

journal = "Gene",

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T1 - Cloning of the human twist gene

T2 - Its expression is retained in adult mesodermally-derived tissues

AU - Wang, Sherry M.

AU - Coljee, Vincent W.

AU - Pignolo, Robert J.

AU - Rotenberg, Mitch O.

AU - Cristofalo, Vincent J.

AU - Sierra, Felipe

N1 - Funding Information: We wish to thank Dr. Marie A. DiBerardino for her helpful discussion and comments. This work was supported by NIH grant AG 00378 to V.J.C.

PY - 1997/3/10

Y1 - 1997/3/10

N2 - We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.

AB - We have previously reported on the isolation of several differentially expressed genes derived from young and senescent non growing WI-38 human fetal lung-derived fibroblasts. A 0.8-kb cDNA clone, isolate EPC-A2 (early population doubling cDNA-A2), encodes the 3' end of the human homolog of the Twist protein. Twist genes encode basic helix-loop-helix DNA-binding transcription factors that play crucial roles in mesoderm development. Here, we report the cloning and sequencing of the genomic human twist gene. It encodes a protein of 201 amino acids with 96% amino acid sequence identity to mouse Twist, and 100% sequence conservation in the DNA-binding region among all species in which it has been characterized. We further show that expression of human twist is retained in mesodermally-derived tissues and cell lines derived from adult donors.

KW - Aging

KW - Genomic DNA

KW - Helix-loop-helix

KW - Tissue-specific expression

KW - Transcription factor

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Cloning of the human twist gene: Its expression is retained in adult mesodermally-derived tissues

Abstract

Keywords

ASJC Scopus subject areas

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