Cloning and sequence analysis of the human gene encoding eosinophil major basic protein

Robert L. Barker, David A. Loegering, Ken C. Arakawa, Larry R. Pease, Gerald J. Gleich

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26 Scopus citations

Abstract

Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.

Original languageEnglish (US)
Pages (from-to)285-289
Number of pages5
JournalGene
Volume86
Issue number2
DOIs
StatePublished - Feb 14 1990

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Keywords

  • Allergy
  • Alu repeat
  • gene polymorphism
  • hypersensitivity
  • pro-protein
  • promoter
  • recombinant DNA
  • toxin

ASJC Scopus subject areas

  • Genetics

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