Cloning and sequence analysis of the human gene encoding eosinophil major basic protein

Robert L. Barker; David A. Loegering; Ken C. Arakawa; Larry R. Pease; Gerald J. Gleich

doi:10.1016/0378-1119(90)90292-Y

Cloning and sequence analysis of the human gene encoding eosinophil major basic protein

Robert L. Barker, David A. Loegering, Ken C. Arakawa, Larry R. Pease, Gerald J. Gleich

Immunology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.

Original language	English (US)
Pages (from-to)	285-289
Number of pages	5
Journal	Gene
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(90)90292-Y
State	Published - Feb 14 1990

Keywords

Allergy
Alu repeat
gene polymorphism
hypersensitivity
pro-protein
promoter
recombinant DNA
toxin

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(90)90292-Y

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title = "Cloning and sequence analysis of the human gene encoding eosinophil major basic protein",

abstract = "Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.",

keywords = "Allergy, Alu repeat, gene polymorphism, hypersensitivity, pro-protein, promoter, recombinant DNA, toxin",

author = "Barker, {Robert L.} and Loegering, {David A.} and Arakawa, {Ken C.} and Pease, {Larry R.} and Gleich, {Gerald J.}",

note = "Funding Information: We thank Linda H. Arneson for preparation of the manuscript. This work was supported in part by grants from the National Institutes of Health (A109728, AI 07047, AI 00706, and A122420) and the Mayo Foundation.",

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doi = "10.1016/0378-1119(90)90292-Y",

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pages = "285--289",

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T1 - Cloning and sequence analysis of the human gene encoding eosinophil major basic protein

AU - Barker, Robert L.

AU - Loegering, David A.

AU - Arakawa, Ken C.

AU - Pease, Larry R.

AU - Gleich, Gerald J.

N1 - Funding Information: We thank Linda H. Arneson for preparation of the manuscript. This work was supported in part by grants from the National Institutes of Health (A109728, AI 07047, AI 00706, and A122420) and the Mayo Foundation.

PY - 1990/2/14

Y1 - 1990/2/14

N2 - Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.

AB - Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.

KW - Allergy

KW - Alu repeat

KW - gene polymorphism

KW - hypersensitivity

KW - pro-protein

KW - promoter

KW - recombinant DNA

KW - toxin

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Cloning and sequence analysis of the human gene encoding eosinophil major basic protein

Abstract

Keywords

ASJC Scopus subject areas

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