Clinicopathologic features of B-cell lineage neoplasms with aberrant expression of CD3

A study of 21 cases

Jennifer Oliveira, Karen L. Grogg, William R. MacOn, Ahmet Dogan, Andrew L Feldman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

CD3 expression by immunohistochemistry was historically considered restricted to T-lineage or NK-lineage neoplasms but recently has been reported in rare cases of mature B-cell neoplasms, frequently in association with Epstein-Barr virus. Here, we describe the pathologic features of 21 B-cell lineage neoplasms that express CD3 protein by immunohistochemistry: 12 diffuse large B-cell lymphomas (DLBCLs); 2 plasmablastic lymphomas (PBLs); 4 plasma cell neoplasms; 2 Burkitt lymphomas; and 1 nodal follicular lymphoma, grade 3A. CD20 expression was negative or only partially positive in 13/21 cases. Epstein-Barr virus was positive in 3/20 tested cases (2 PBLs and 1 DLBCL). All tested neoplasms (14/14) had clonal immunoglobulin gene rearrangements, and no clonal T-cell gene rearrangements were detected (0/14). The 12 DLBCLs segregated into 2 main groups: 7 demonstrated features of plasmacytic differentiation but did not meet criteria for PBL, and 5 had anaplastic features. In addition to morphology, other features shared among the DLBCLs with plasmacytic differentiation, the plasma cell neoplasms, and the PBLs included extranodal presentation, cytoplasmic localization of CD3, and lack of expression of other T-cell antigens in most cases. In contrast, DLBCLs with anaplastic features and the single follicular lymphoma coexpressed multiple T-cell antigens in a predominantly membranous pattern and presented with nodal disease in a relatively younger patient population. Our data expand the spectrum of morphologic, phenotypic, and clinical features of B-cell neoplasms aberrantly expressing CD3. As these neoplasms often lack typical expression of B-cell antigens, knowledge of these features will help avoid misclassification.

Original languageEnglish (US)
Pages (from-to)1364-1370
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume36
Issue number9
DOIs
StatePublished - Sep 2012

Fingerprint

Cell Lineage
Lymphoma, Large B-Cell, Diffuse
B-Lymphocytes
Plasma Cell Neoplasms
Neoplasms
Follicular Lymphoma
Viral Tumor Antigens
Human Herpesvirus 4
Immunohistochemistry
T-Lymphocyte Gene Rearrangement
T-Lymphocytes
Immunoglobulin Genes
Burkitt Lymphoma
Gene Rearrangement
B-Cell Lymphoma
Antigens
Plasmablastic Lymphoma
Population
Proteins

Keywords

  • aberrant T-cell antigen
  • B-cell lymphoma
  • Burkitt
  • CD3
  • DLBCL
  • follicular
  • lineage plasticity
  • plasma cell myeloma
  • plasmablastic

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

Clinicopathologic features of B-cell lineage neoplasms with aberrant expression of CD3 : A study of 21 cases. / Oliveira, Jennifer; Grogg, Karen L.; MacOn, William R.; Dogan, Ahmet; Feldman, Andrew L.

In: American Journal of Surgical Pathology, Vol. 36, No. 9, 09.2012, p. 1364-1370.

Research output: Contribution to journalArticle

@article{72f6c1e93f6346d3b7240cc55df3dc5c,
title = "Clinicopathologic features of B-cell lineage neoplasms with aberrant expression of CD3: A study of 21 cases",
abstract = "CD3 expression by immunohistochemistry was historically considered restricted to T-lineage or NK-lineage neoplasms but recently has been reported in rare cases of mature B-cell neoplasms, frequently in association with Epstein-Barr virus. Here, we describe the pathologic features of 21 B-cell lineage neoplasms that express CD3 protein by immunohistochemistry: 12 diffuse large B-cell lymphomas (DLBCLs); 2 plasmablastic lymphomas (PBLs); 4 plasma cell neoplasms; 2 Burkitt lymphomas; and 1 nodal follicular lymphoma, grade 3A. CD20 expression was negative or only partially positive in 13/21 cases. Epstein-Barr virus was positive in 3/20 tested cases (2 PBLs and 1 DLBCL). All tested neoplasms (14/14) had clonal immunoglobulin gene rearrangements, and no clonal T-cell gene rearrangements were detected (0/14). The 12 DLBCLs segregated into 2 main groups: 7 demonstrated features of plasmacytic differentiation but did not meet criteria for PBL, and 5 had anaplastic features. In addition to morphology, other features shared among the DLBCLs with plasmacytic differentiation, the plasma cell neoplasms, and the PBLs included extranodal presentation, cytoplasmic localization of CD3, and lack of expression of other T-cell antigens in most cases. In contrast, DLBCLs with anaplastic features and the single follicular lymphoma coexpressed multiple T-cell antigens in a predominantly membranous pattern and presented with nodal disease in a relatively younger patient population. Our data expand the spectrum of morphologic, phenotypic, and clinical features of B-cell neoplasms aberrantly expressing CD3. As these neoplasms often lack typical expression of B-cell antigens, knowledge of these features will help avoid misclassification.",
keywords = "aberrant T-cell antigen, B-cell lymphoma, Burkitt, CD3, DLBCL, follicular, lineage plasticity, plasma cell myeloma, plasmablastic",
author = "Jennifer Oliveira and Grogg, {Karen L.} and MacOn, {William R.} and Ahmet Dogan and Feldman, {Andrew L}",
year = "2012",
month = "9",
doi = "10.1097/PAS.0b013e31825e63a9",
language = "English (US)",
volume = "36",
pages = "1364--1370",
journal = "American Journal of Surgical Pathology",
issn = "0147-5185",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Clinicopathologic features of B-cell lineage neoplasms with aberrant expression of CD3

T2 - A study of 21 cases

AU - Oliveira, Jennifer

AU - Grogg, Karen L.

AU - MacOn, William R.

AU - Dogan, Ahmet

AU - Feldman, Andrew L

PY - 2012/9

Y1 - 2012/9

N2 - CD3 expression by immunohistochemistry was historically considered restricted to T-lineage or NK-lineage neoplasms but recently has been reported in rare cases of mature B-cell neoplasms, frequently in association with Epstein-Barr virus. Here, we describe the pathologic features of 21 B-cell lineage neoplasms that express CD3 protein by immunohistochemistry: 12 diffuse large B-cell lymphomas (DLBCLs); 2 plasmablastic lymphomas (PBLs); 4 plasma cell neoplasms; 2 Burkitt lymphomas; and 1 nodal follicular lymphoma, grade 3A. CD20 expression was negative or only partially positive in 13/21 cases. Epstein-Barr virus was positive in 3/20 tested cases (2 PBLs and 1 DLBCL). All tested neoplasms (14/14) had clonal immunoglobulin gene rearrangements, and no clonal T-cell gene rearrangements were detected (0/14). The 12 DLBCLs segregated into 2 main groups: 7 demonstrated features of plasmacytic differentiation but did not meet criteria for PBL, and 5 had anaplastic features. In addition to morphology, other features shared among the DLBCLs with plasmacytic differentiation, the plasma cell neoplasms, and the PBLs included extranodal presentation, cytoplasmic localization of CD3, and lack of expression of other T-cell antigens in most cases. In contrast, DLBCLs with anaplastic features and the single follicular lymphoma coexpressed multiple T-cell antigens in a predominantly membranous pattern and presented with nodal disease in a relatively younger patient population. Our data expand the spectrum of morphologic, phenotypic, and clinical features of B-cell neoplasms aberrantly expressing CD3. As these neoplasms often lack typical expression of B-cell antigens, knowledge of these features will help avoid misclassification.

AB - CD3 expression by immunohistochemistry was historically considered restricted to T-lineage or NK-lineage neoplasms but recently has been reported in rare cases of mature B-cell neoplasms, frequently in association with Epstein-Barr virus. Here, we describe the pathologic features of 21 B-cell lineage neoplasms that express CD3 protein by immunohistochemistry: 12 diffuse large B-cell lymphomas (DLBCLs); 2 plasmablastic lymphomas (PBLs); 4 plasma cell neoplasms; 2 Burkitt lymphomas; and 1 nodal follicular lymphoma, grade 3A. CD20 expression was negative or only partially positive in 13/21 cases. Epstein-Barr virus was positive in 3/20 tested cases (2 PBLs and 1 DLBCL). All tested neoplasms (14/14) had clonal immunoglobulin gene rearrangements, and no clonal T-cell gene rearrangements were detected (0/14). The 12 DLBCLs segregated into 2 main groups: 7 demonstrated features of plasmacytic differentiation but did not meet criteria for PBL, and 5 had anaplastic features. In addition to morphology, other features shared among the DLBCLs with plasmacytic differentiation, the plasma cell neoplasms, and the PBLs included extranodal presentation, cytoplasmic localization of CD3, and lack of expression of other T-cell antigens in most cases. In contrast, DLBCLs with anaplastic features and the single follicular lymphoma coexpressed multiple T-cell antigens in a predominantly membranous pattern and presented with nodal disease in a relatively younger patient population. Our data expand the spectrum of morphologic, phenotypic, and clinical features of B-cell neoplasms aberrantly expressing CD3. As these neoplasms often lack typical expression of B-cell antigens, knowledge of these features will help avoid misclassification.

KW - aberrant T-cell antigen

KW - B-cell lymphoma

KW - Burkitt

KW - CD3

KW - DLBCL

KW - follicular

KW - lineage plasticity

KW - plasma cell myeloma

KW - plasmablastic

UR - http://www.scopus.com/inward/record.url?scp=84865525718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865525718&partnerID=8YFLogxK

U2 - 10.1097/PAS.0b013e31825e63a9

DO - 10.1097/PAS.0b013e31825e63a9

M3 - Article

VL - 36

SP - 1364

EP - 1370

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

SN - 0147-5185

IS - 9

ER -