Clathrin regulates the association of PIPKIγ661 with the AP-2 adaptor β2 Appendage

James R. Thieman, Sanjay K. Mishra, Kun Ling, Balraj Doray, Richard A. Anderson, Linton M. Traub

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The AP-2 clathrin adaptor differs fundamentally from the related AP-1, AP-3, and AP-4 sorting complexes because membrane deposition does not depend directly on an Arf family GTPase. Instead phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) appears to act as the principal compartmental cue for AP-2 placement at the plasma membrane as well as for the docking of numerous other important clathrin coat components at the nascent bud site. This PtdIns(4,5)P2 dependence makes type I phosphatidylinositol 4-phosphate 5-kinases (PIPKIs) lynchpin enzymes in the assembly of clathrin-coated structures at the cell surface. PIPKIγ is the chief 5-kinase at nerve terminals, and here we show that the 26-amino acid, alternatively spliced C terminus of PIPKIγ661 is an intrinsically unstructured polypeptide that binds directly to the sandwich subdomain of the AP-2 β2 subunit appendage. An aromatic side chain-based, extended interaction motif that also includes the two bulky C-terminal residues of the short PIPKIγ635 variant is necessary for β2 appendage engagement. The clathrin heavy chain accesses the same contact surface on the AP-2b2 appendage, but because of additional clathrin binding sites located within the unstructured hinge segment of the β2 subunit, clathrin binds the β2 chain with a higher apparent affinity than PIPKIγ661. A clathrin-regulated interaction with AP-2 could allow PIPKIγ661 to be strategically positioned for regional PtdIns(4,5)P2 generation during clathrin-coated vesicle assembly at the synapse.

Original languageEnglish (US)
Pages (from-to)13924-13939
Number of pages16
JournalJournal of Biological Chemistry
Volume284
Issue number20
DOIs
StatePublished - May 15 2009

Fingerprint

Clathrin
Phosphatidylinositol 4,5-Diphosphate
Clathrin Heavy Chains
Vesicular Transport Adaptor Proteins
Clathrin-Coated Vesicles
GTP Phosphohydrolases
Transcription Factor AP-1
Phosphatidylinositols
Synapses
Cues
Hinges
Cell membranes
Sorting
Phosphotransferases
Binding Sites
Cell Membrane
Amino Acids
Peptides
Membranes
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Clathrin regulates the association of PIPKIγ661 with the AP-2 adaptor β2 Appendage. / Thieman, James R.; Mishra, Sanjay K.; Ling, Kun; Doray, Balraj; Anderson, Richard A.; Traub, Linton M.

In: Journal of Biological Chemistry, Vol. 284, No. 20, 15.05.2009, p. 13924-13939.

Research output: Contribution to journalArticle

Thieman, James R. ; Mishra, Sanjay K. ; Ling, Kun ; Doray, Balraj ; Anderson, Richard A. ; Traub, Linton M. / Clathrin regulates the association of PIPKIγ661 with the AP-2 adaptor β2 Appendage. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 20. pp. 13924-13939.
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