TY - JOUR
T1 - Circulating tumor cell analysis in metastatic triple-negative breast cancers
AU - Magbanua, Mark Jesus M.
AU - Carey, Lisa A.
AU - DeLuca, Amy
AU - Hwang, Jimmy
AU - Scott, Janet H.
AU - Rimawi, Mothaffar F.
AU - Mayer, Erica L.
AU - Marcom, P. Kelly
AU - Liu, Minetta C.
AU - Esteva, Francisco J.
AU - Park, John W.
AU - Rugo, Hope S.
N1 - Publisher Copyright:
© 2014 AACR.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Purpose: Recent developments in rare-cell technology have led to improved blood-based assays that allow for the reliable detection, enumeration, and more recently, genomic profiling of circulating tumor cells (CTC). We evaluated two different approaches for enumeration of CTCs in a prospective therapeutic study of patients with metastatic triple-negative breast cancer (TNBC). Experimental Design: The CellSearch system, a commercially available and U.S. Food and Drug Administration (FDA)-cleared assay for CTC enumeration, and IE/FC, an alternative method using EPCAM-based immunomagnetic enrichment and flow cytometry that maintains cell viability, were used to enumerate CTCs in the blood of patients with metastatic TNBC. CTC numbers were assessed at baseline and 7 to 14 days after initiation of therapy with cetuximab-carboplatin in a phase II multicenter clinical trial (TBCRC 001). Results: CTC numbers from two methods were significantly correlated at baseline (r =0.62) and at 7 to 14 days (r =0.53). Baseline CTCs showed no association with time-to-progression (TTP), whereas CTCs at 7 to 14 days were significantly correlated with TTP (CellSearch P =0.02; IE/FC P =0.03). CTCs at both time points were significantly associated with overall survival (OS) [CellSearch: baseline (P =0.0001) and 7 to 14 days (P < 0.0001); IE/FC: baseline (P =0.0009) and 7 to 14 days (P =0.0086)]. Conclusions: Our findings demonstrate that CTC enumeration by two different assays was highly concordant. In addition, results of both assays were significantly correlated with TTP and OS in patients with TNBC. The IE/FC method is also easily adapted to isolation of pure populations of CTCs for genomic profiling. Clin Cancer Res; 21(5); 1098-105.
AB - Purpose: Recent developments in rare-cell technology have led to improved blood-based assays that allow for the reliable detection, enumeration, and more recently, genomic profiling of circulating tumor cells (CTC). We evaluated two different approaches for enumeration of CTCs in a prospective therapeutic study of patients with metastatic triple-negative breast cancer (TNBC). Experimental Design: The CellSearch system, a commercially available and U.S. Food and Drug Administration (FDA)-cleared assay for CTC enumeration, and IE/FC, an alternative method using EPCAM-based immunomagnetic enrichment and flow cytometry that maintains cell viability, were used to enumerate CTCs in the blood of patients with metastatic TNBC. CTC numbers were assessed at baseline and 7 to 14 days after initiation of therapy with cetuximab-carboplatin in a phase II multicenter clinical trial (TBCRC 001). Results: CTC numbers from two methods were significantly correlated at baseline (r =0.62) and at 7 to 14 days (r =0.53). Baseline CTCs showed no association with time-to-progression (TTP), whereas CTCs at 7 to 14 days were significantly correlated with TTP (CellSearch P =0.02; IE/FC P =0.03). CTCs at both time points were significantly associated with overall survival (OS) [CellSearch: baseline (P =0.0001) and 7 to 14 days (P < 0.0001); IE/FC: baseline (P =0.0009) and 7 to 14 days (P =0.0086)]. Conclusions: Our findings demonstrate that CTC enumeration by two different assays was highly concordant. In addition, results of both assays were significantly correlated with TTP and OS in patients with TNBC. The IE/FC method is also easily adapted to isolation of pure populations of CTCs for genomic profiling. Clin Cancer Res; 21(5); 1098-105.
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U2 - 10.1158/1078-0432.CCR-14-1948
DO - 10.1158/1078-0432.CCR-14-1948
M3 - Article
C2 - 25524311
AN - SCOPUS:84927728470
SN - 1078-0432
VL - 21
SP - 1098
EP - 1105
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 5
ER -