Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations

Hideyiiki Sasaki; Mikihiro Kihara; Paula J. Zollman; Kim K. Nickander; Inge L. Smithson; James D. Schmelzer; Catherine L. Willner; Eduardo E. Benarroch; Phillip A. Low

doi:10.1007/s004010050583

Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations

Hideyiiki Sasaki, Mikihiro Kihara, Paula J. Zollman, Kim K. Nickander, Inge L. Smithson, James D. Schmelzer, Catherine L. Willner, Eduardo E. Benarroch, Phillip A. Low

Neurology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.

Original language	English (US)
Pages (from-to)	62-70
Number of pages	9
Journal	Acta neuropathologica
Volume	93
Issue number	1
DOIs	https://doi.org/10.1007/s004010050583
State	Published - Dec 1996

Keywords

Adrenergic
Calcitonin gene-related peptide
Ischemia
Pain
Sciatic nerve

ASJC Scopus subject areas

Pathology and Forensic Medicine
Clinical Neurology
Cellular and Molecular Neuroscience

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10.1007/s004010050583

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@article{913094f81c6944e9b2061833387c2ac8,

title = "Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations",

abstract = "We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.",

keywords = "Adrenergic, Calcitonin gene-related peptide, Ischemia, Pain, Sciatic nerve",

author = "Hideyiiki Sasaki and Mikihiro Kihara and Zollman, {Paula J.} and Nickander, {Kim K.} and Smithson, {Inge L.} and Schmelzer, {James D.} and Willner, {Catherine L.} and Benarroch, {Eduardo E.} and Low, {Phillip A.}",

year = "1996",

month = dec,

doi = "10.1007/s004010050583",

language = "English (US)",

volume = "93",

pages = "62--70",

journal = "Acta neuropathologica",

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T1 - Chronic constriction model of rat sciatic nerve

T2 - Nerve blood flow, morphologic and biochemical alterations

AU - Sasaki, Hideyiiki

AU - Kihara, Mikihiro

AU - Zollman, Paula J.

AU - Nickander, Kim K.

AU - Smithson, Inge L.

AU - Schmelzer, James D.

AU - Willner, Catherine L.

AU - Benarroch, Eduardo E.

AU - Low, Phillip A.

PY - 1996/12

Y1 - 1996/12

N2 - We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.

AB - We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.

KW - Adrenergic

KW - Calcitonin gene-related peptide

KW - Ischemia

KW - Pain

KW - Sciatic nerve

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Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations

Abstract

Keywords

ASJC Scopus subject areas

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