Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations

Hideyiiki Sasaki, Mikihiro Kihara, Paula J. Zollman, Kim K. Nickander, Inge L. Smithson, James D. Schmelzer, Catherine L. Willner, Eduardo E. Benarroch, Phillip A. Low

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We evaluated the nerve blood flow (NBF), light and electron microscopy, and adrenergic innervation of rat sciatic nerve at 2-45 days after the application of four loose ligatures. Ischemia developed at the lesion edge, creating an endoneurial dam. Calcitonin gene-related peptide, norepinephrine and NBF were increased within the lesion. Morphologic alterations consisted of early endoneurial edema, followed by myelinated fiber degeneration, with relative sparing of small myelinated and unmyelinated nerve fibers, and leukocyte adhesion to microvessels. Axonal degeneration predominated over demyelination. At 45 days, profuse regeneration of small myelinated fibers was seen. The mechanism of lesional sensitization is discussed.

Original languageEnglish (US)
Pages (from-to)62-70
Number of pages9
JournalActa neuropathologica
Volume93
Issue number1
DOIs
StatePublished - Dec 1996

Keywords

  • Adrenergic
  • Calcitonin gene-related peptide
  • Ischemia
  • Pain
  • Sciatic nerve

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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    Sasaki, H., Kihara, M., Zollman, P. J., Nickander, K. K., Smithson, I. L., Schmelzer, J. D., Willner, C. L., Benarroch, E. E., & Low, P. A. (1996). Chronic constriction model of rat sciatic nerve: Nerve blood flow, morphologic and biochemical alterations. Acta neuropathologica, 93(1), 62-70. https://doi.org/10.1007/s004010050583