Chromosome anomalies detected by interphase fluorescence in situ hybridization: Correlation with significant biological features of B-cell chronic lymphocytic leukaemia

Gordon W. Dewald, Stephanie R. Brockman, Sarah F. Paternoster, Nancy D. Bone, Judith R. O'Fallon, Cristine Allmer, Charles D. James, Diane F Jelinek, Renee C. Tschumper, Curtis A. Hanson, Rajiv K. Pruthi, Thomas Elmer Witzig, Timothy G. Call, Neil Elliot Kay

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) was used to detect 6q-, 11q-, +12, 13q-, 17p- and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B-cell chronic lymphocytic leukaemia (B-CLL). A total of 87 patients (77%) had a FISH anomaly: 13q- × 1 was most frequent (64%) followed by 13q- × 2 (28%), +12 (25%), 11q-(15%), 17p- (8%) and 6q- (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5+/CD19+ cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38+ expression and IgVH mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38+, but not by age, sex or IgVH mutational status. To look for baseline factors associated with high-risk disease, multivariate analysis of age, sex, Rai stage, CD38+ and disease status versus FISH risk category was performed. Importantly, only CD38+ was significantly associated with high-risk FISH categories (+12, 11q- and 17p-) after adjustment for the effects of other variables.

Original languageEnglish (US)
Pages (from-to)287-295
Number of pages9
JournalBritish Journal of Haematology
Volume121
Issue number2
DOIs
StatePublished - Apr 2003

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Interphase
B-Cell Chronic Lymphocytic Leukemia
Fluorescence In Situ Hybridization
Chromosomes
Bone Marrow Cells
Blood Cells
B-Lymphocytes
Multivariate Analysis
Bone Marrow

Keywords

  • B-CLL
  • CD38 expression
  • FISH
  • IgV mutations
  • Rai stage

ASJC Scopus subject areas

  • Hematology

Cite this

Chromosome anomalies detected by interphase fluorescence in situ hybridization : Correlation with significant biological features of B-cell chronic lymphocytic leukaemia. / Dewald, Gordon W.; Brockman, Stephanie R.; Paternoster, Sarah F.; Bone, Nancy D.; O'Fallon, Judith R.; Allmer, Cristine; James, Charles D.; Jelinek, Diane F; Tschumper, Renee C.; Hanson, Curtis A.; Pruthi, Rajiv K.; Witzig, Thomas Elmer; Call, Timothy G.; Kay, Neil Elliot.

In: British Journal of Haematology, Vol. 121, No. 2, 04.2003, p. 287-295.

Research output: Contribution to journalArticle

Dewald, Gordon W. ; Brockman, Stephanie R. ; Paternoster, Sarah F. ; Bone, Nancy D. ; O'Fallon, Judith R. ; Allmer, Cristine ; James, Charles D. ; Jelinek, Diane F ; Tschumper, Renee C. ; Hanson, Curtis A. ; Pruthi, Rajiv K. ; Witzig, Thomas Elmer ; Call, Timothy G. ; Kay, Neil Elliot. / Chromosome anomalies detected by interphase fluorescence in situ hybridization : Correlation with significant biological features of B-cell chronic lymphocytic leukaemia. In: British Journal of Haematology. 2003 ; Vol. 121, No. 2. pp. 287-295.
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AU - Dewald, Gordon W.

AU - Brockman, Stephanie R.

AU - Paternoster, Sarah F.

AU - Bone, Nancy D.

AU - O'Fallon, Judith R.

AU - Allmer, Cristine

AU - James, Charles D.

AU - Jelinek, Diane F

AU - Tschumper, Renee C.

AU - Hanson, Curtis A.

AU - Pruthi, Rajiv K.

AU - Witzig, Thomas Elmer

AU - Call, Timothy G.

AU - Kay, Neil Elliot

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AB - Fluorescence in situ hybridization (FISH) was used to detect 6q-, 11q-, +12, 13q-, 17p- and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B-cell chronic lymphocytic leukaemia (B-CLL). A total of 87 patients (77%) had a FISH anomaly: 13q- × 1 was most frequent (64%) followed by 13q- × 2 (28%), +12 (25%), 11q-(15%), 17p- (8%) and 6q- (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5+/CD19+ cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38+ expression and IgVH mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38+, but not by age, sex or IgVH mutational status. To look for baseline factors associated with high-risk disease, multivariate analysis of age, sex, Rai stage, CD38+ and disease status versus FISH risk category was performed. Importantly, only CD38+ was significantly associated with high-risk FISH categories (+12, 11q- and 17p-) after adjustment for the effects of other variables.

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