Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum

Yanhui Lu, Yoonseong Park, Xiwu Gao, Xin Zhang, Jianxiu Yao, Yuan-Ping Pang, Haobo Jiang, Kun Yan Zhu

Research output: Contribution to journalArticle

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Abstract

We compared biological functions of two acetylcholinesterase genes (TcAce1 and TcAce2) in Tribolium castaneum, a globally distributed major pest of stored grain products and an emerging model organism, by using RNA interference. Although both genes expressed at all developmental stages and mainly in the brain, the transcript level of TcAce1 was 1.2-to 8.7-fold higher than that of TcAce2, depending on developmental stages. Silencing TcAce1 in 20-day larvae led to 100% mortality within two weeks after eclosion and increased larval susceptibilities to anticholinesterase insecticides. In contrast, silencing TcAce2 did not show insect mortality and significantly affect insecticide susceptibility, but delayed insect development and reduced female egg-laying and egg hatching. These results demonstrate for the first time that TcAce1 plays a major role in cholinergic functions and is the target of anticholinesterase insecticides, whereas TcAce2 plays an important, non-cholinergic role in female reproduction, embryo development, and growth of offspring.

Original languageEnglish (US)
Article number288
JournalScientific Reports
Volume2
DOIs
StatePublished - 2012

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Tribolium
Gene Silencing
Acetylcholinesterase
Insecticides
Cholinergic Agents
Cholinesterase Inhibitors
Ovum
Insects
Genes
Mortality
RNA Interference
Embryonic Development
Reproduction
Larva
Brain
Growth

ASJC Scopus subject areas

  • General

Cite this

Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum. / Lu, Yanhui; Park, Yoonseong; Gao, Xiwu; Zhang, Xin; Yao, Jianxiu; Pang, Yuan-Ping; Jiang, Haobo; Zhu, Kun Yan.

In: Scientific Reports, Vol. 2, 288, 2012.

Research output: Contribution to journalArticle

Lu, Yanhui ; Park, Yoonseong ; Gao, Xiwu ; Zhang, Xin ; Yao, Jianxiu ; Pang, Yuan-Ping ; Jiang, Haobo ; Zhu, Kun Yan. / Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum. In: Scientific Reports. 2012 ; Vol. 2.
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