Characterization of the three-compartment gel-entrapment porcine hepatocyte bioartificial liver

T. D. Sielaff, Scott Nyberg, M. D. Rollins, M. Y. Hu, B. Amiot, A. Lee, F. J. Wu, W. S. Hu, F. B. Cerra

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A hybrid bioartificial liver device supporting a large mass of cells expressing differentiated hepatocyte metabolic capabilities is necessary for the successful treatment of fulminant hepatic failure. The three-compartment gel-entrapment porcine hepatocyte bioartificial liver was designed to provide 'bridge' support to transplantation or until native liver recovery is achieved for patients with acute liver failure. The device is an automated mammalian cell culture system supporting 6-7 x 109 porcine hepatocytes entrapped in a collagen matrix and inoculated into the capillary lumen spaces of two 100 kDa molecular mass cut-off hollow fiber bioreactors. Gel contraction recreates a small lumen space within the hollow fiber which allows for the delivery of a nutrient medium. This configuration supported hepatocyte viability and differentiated phenotype as measured by albumin synthesis, ureagenesis, oxygen consumption, and vital dye staining during both cell culture and ex vivo application The hollow fiber membrane was also shown to isolate the cells from xenogenic immunoglobulin attack. The gel-entrapment bioartificial liver maintained a large mass of functional hepatocytes by providing a three-dimensional cell culture matrix, by delivering basal nutrients through lumen media perfusion, and by preventing rejection of the xenocytes. These features make this device a favorable candidate for the treatment of clinical fulminant hepatic failure.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalCell Biology and Toxicology
Volume13
Issue number4-5
DOIs
StatePublished - Sep 16 1997

Fingerprint

Artificial Liver
Liver
Hepatocytes
Swine
Gels
Acute Liver Failure
Cell culture
Cell Culture Techniques
Equipment and Supplies
Nutrients
Fibers
Food
Molecular mass
Bioreactors
Oxygen Consumption
Immunoglobulins
Albumins
Coloring Agents
Collagen
Perfusion

Keywords

  • Artificial organ
  • Bioartificial liver
  • Porcine hepatocyte

ASJC Scopus subject areas

  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

Cite this

Characterization of the three-compartment gel-entrapment porcine hepatocyte bioartificial liver. / Sielaff, T. D.; Nyberg, Scott; Rollins, M. D.; Hu, M. Y.; Amiot, B.; Lee, A.; Wu, F. J.; Hu, W. S.; Cerra, F. B.

In: Cell Biology and Toxicology, Vol. 13, No. 4-5, 16.09.1997, p. 357-364.

Research output: Contribution to journalArticle

Sielaff, TD, Nyberg, S, Rollins, MD, Hu, MY, Amiot, B, Lee, A, Wu, FJ, Hu, WS & Cerra, FB 1997, 'Characterization of the three-compartment gel-entrapment porcine hepatocyte bioartificial liver', Cell Biology and Toxicology, vol. 13, no. 4-5, pp. 357-364. https://doi.org/10.1023/A:1007499727772
Sielaff, T. D. ; Nyberg, Scott ; Rollins, M. D. ; Hu, M. Y. ; Amiot, B. ; Lee, A. ; Wu, F. J. ; Hu, W. S. ; Cerra, F. B. / Characterization of the three-compartment gel-entrapment porcine hepatocyte bioartificial liver. In: Cell Biology and Toxicology. 1997 ; Vol. 13, No. 4-5. pp. 357-364.
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