TY - JOUR
T1 - Characterization of the antigenic structure of human type II collagen
AU - Krco, Christopher J.
AU - Pawelski, Jeff
AU - Harders, Jerry
AU - McCormick, Daniel
AU - Griffiths, Marie
AU - Luthra, Harvindar S.
AU - David, Chella S.
PY - 1996/4/15
Y1 - 1996/4/15
N2 - A series of 101 peptides each 20 amino acids in length (10-residue overlap) spanning the helical portion of the mature α-chain of human type II collagen (CII) was synthesized. DBA/1 (H-2q) mice were immunized with individual peptides, and draining lymph node cells were challenged in vitro. Strong responses were measured to three peptides: peptide I (residues 74-93), peptide 14 (residues 254-273), and peptide 81 (residues 924-943), B10.Q (H-2q) mice were responsive to peptides I and 81 but not to peptide 14. B10.RIII (H-2r) mice, which are resistant to arthritis induction following immunization with human CII, were unresponsive to peptides I, 14, and 81. Using single amino acid truncated peptides, we determined minimal immunostimulatory lengths for peptides I and 81. Residues critical to antigenicity were identified by introducing alanine and glycine substitutions into minimal length immunostimuiatory peptides. The determinants within peptides I and 81 are 100% homologous to mouse CU and are autoantigens. Peptide 81 has homology to viral proteins. Peptide 14 is 90% homologous to mouse CII and has homology to heat shock proteins.
AB - A series of 101 peptides each 20 amino acids in length (10-residue overlap) spanning the helical portion of the mature α-chain of human type II collagen (CII) was synthesized. DBA/1 (H-2q) mice were immunized with individual peptides, and draining lymph node cells were challenged in vitro. Strong responses were measured to three peptides: peptide I (residues 74-93), peptide 14 (residues 254-273), and peptide 81 (residues 924-943), B10.Q (H-2q) mice were responsive to peptides I and 81 but not to peptide 14. B10.RIII (H-2r) mice, which are resistant to arthritis induction following immunization with human CII, were unresponsive to peptides I, 14, and 81. Using single amino acid truncated peptides, we determined minimal immunostimulatory lengths for peptides I and 81. Residues critical to antigenicity were identified by introducing alanine and glycine substitutions into minimal length immunostimuiatory peptides. The determinants within peptides I and 81 are 100% homologous to mouse CU and are autoantigens. Peptide 81 has homology to viral proteins. Peptide 14 is 90% homologous to mouse CII and has homology to heat shock proteins.
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M3 - Article
C2 - 8609394
AN - SCOPUS:0030584696
SN - 0022-1767
VL - 156
SP - 2761
EP - 2768
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -