Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

Nora E. Torres; Paula J. Zollman; Phillip A. Low

doi:10.1016/0006-8993(91)90415-R

Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

Nora E. Torres, Paula J. Zollman, Phillip A. Low

Neurology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [³H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M₁ (pirenzepine), M₂ (AF-DX 116), and M₃ (4-DAMP) and the classical nonselective antagonist atropine. pK_i for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M₃.

Original language	English (US)
Pages (from-to)	129-132
Number of pages	4
Journal	Brain Research
Volume	550
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(91)90415-R
State	Published - May 31 1991

Keywords

Autoradiography
Eccrine
Muscarinic
Rat
Sweat Gland

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)90415-R

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title = "Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography",

abstract = "The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.",

keywords = "Autoradiography, Eccrine, Muscarinic, Rat, Sweat Gland",

author = "Torres, {Nora E.} and Zollman, {Paula J.} and Low, {Phillip A.}",

note = "Funding Information: Supportedin part by grants from NINCDS (ROI NS22352, NS14302,N O1 NS72302),M DA, and Mayo Funds. P.A.L. is the recipienot f a Jacob Javits NeurosciencIen vestigatoArw ard.",

year = "1991",

month = may,

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doi = "10.1016/0006-8993(91)90415-R",

language = "English (US)",

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T1 - Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

AU - Torres, Nora E.

AU - Zollman, Paula J.

AU - Low, Phillip A.

N1 - Funding Information: Supportedin part by grants from NINCDS (ROI NS22352, NS14302,N O1 NS72302),M DA, and Mayo Funds. P.A.L. is the recipienot f a Jacob Javits NeurosciencIen vestigatoArw ard.

PY - 1991/5/31

Y1 - 1991/5/31

N2 - The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.

AB - The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.

KW - Autoradiography

KW - Eccrine

KW - Muscarinic

KW - Rat

KW - Sweat Gland

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U2 - 10.1016/0006-8993(91)90415-R

DO - 10.1016/0006-8993(91)90415-R

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AN - SCOPUS:0025808776

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EP - 132

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

Abstract

Keywords

ASJC Scopus subject areas

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