Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

Nora E. Torres, Paula J. Zollman, Phillip A. Low

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalBrain Research
Volume550
Issue number1
DOIs
StatePublished - May 31 1991

Keywords

  • Autoradiography
  • Eccrine
  • Muscarinic
  • Rat
  • Sweat Gland

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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