Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography

Nora E. Torres, Paula J. Zollman, Phillip Anson Low

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine. pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalBrain Research
Volume550
Issue number1
DOIs
StatePublished - May 31 1991

Fingerprint

Eccrine Glands
Pirenzepine
Sweat Glands
Muscarinic Receptors
Autoradiography
Atropine
Cholinergic Receptors
Binding Sites
4-diphenylacetoxy-1,1-dimethylpiperidinium
otenzepad

Keywords

  • Autoradiography
  • Eccrine
  • Muscarinic
  • Rat
  • Sweat Gland

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography. / Torres, Nora E.; Zollman, Paula J.; Low, Phillip Anson.

In: Brain Research, Vol. 550, No. 1, 31.05.1991, p. 129-132.

Research output: Contribution to journalArticle

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