Changes in nucleosome occupancy occur in a chromosome specific manner

Brittany S. Sexton, Brooke R. Druliner, Denis Avey, Fanxiu Zhu, Jonathan H. Dennis

Research output: Contribution to journalArticlepeer-review

Abstract

In the eukaryotic nucleus, DNA is packaged into chromatin. The fundamental subunit of chromatin is the nucleosome, DNA is wrapped 1.6 times around a histone octamer core. Nuclear processes in eukaryotes are impacted by whether regulatory DNA is occupied by nucleosomes. We used microarrays to measure nucleosome occupancy in human cells post-Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation at hundreds of immunity-related loci. The detailed analysis of these technologies can be found in recent publications from our lab [1,3]. We found that nucleosome redistributions displayed chromosome specific nucleosome occupancy. This resource can be used to map nucleosome distributions in a variety of biological contexts.

Original languageEnglish (US)
Pages (from-to)114-116
Number of pages3
JournalGenomics Data
Volume2
DOIs
StatePublished - 2014

Keywords

  • KSHV
  • Microarray
  • MNase
  • Nucleosome

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

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