CGH protocols: Chronic lymphocytic leukemia

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Array-based comparative genomic hybridization (aCGH) is a powerful assay to identify copy number abnormalities underlying the pathogenesis of cancer. aCGH has become the gold standard for whole genome copy number analysis in medium and large cohorts in clinical and research laboratories. Identifying the best workflow is critical to achieving the optimal performance for this assay. Here we describe the aCGH protocol used by our group in the study of B-chronic lymphocytic leukemia (CLL). We also describe some initial applications of aCGH in association with clinical outcome for CLL.

Original languageEnglish (US)
Title of host publicationArray Comparative Genomic Hybridization
Subtitle of host publicationProtocols and Applications
PublisherHumana Press Inc.
Pages87-98
Number of pages12
ISBN (Print)9781627032803
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume973
ISSN (Print)1064-3745

Keywords

  • ACGH
  • Cll
  • DNA digestion
  • DNA labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Braggio, E., Fonseca, R., & Kay, N. E. (2013). CGH protocols: Chronic lymphocytic leukemia. In Array Comparative Genomic Hybridization: Protocols and Applications (pp. 87-98). (Methods in Molecular Biology; Vol. 973). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-281-0_6