Centrin is a component of the periocentriola lattice

Andre T. Baron, Tammy M. Greenwood, Christopher W. Bazinet, Jeffrey L. Salisbury

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Here, we use three polyclonal anticentrin antisera designated 08/28, 26/14-1, and 26/14-2 to further characterize the pericentriolar lattice of metazoan cells. all of these antibodies give an indistinguishable localization pattern that consists of a constellation of pericentrosomal spots. In QT6 cells these spots are few in number and closely associated with the centriolar region, whereas in PtK2 cells they are more numerous and distributed further from the point of microtubule focus. In mitotic cells, centrin is localized to the spindle poles and spindle apparatus. We demonstrate here that the pericentriolar lattice of PtK2 and QT6 cells is, in part, composed of proteins characterized by acidic pIs (4.4 to 5.4), low molecular mass (Mr 18 500-21 000), and calcium-binding; these attributes and the immunoreactivity of these proteins to anticentrin antibodies indicate that they are centrin isoforms of metazoan cells. Finally, we confirm our earlier observation that PtK2 cells contain a centrin-related protein of Mr 165 000; QT6 cells also contain centrin-related proteins (Mr 64 000-165 000). We conclude that centrin is a component of the pericentriolar lattice of higher eukaryotic centrosomes.

Original languageEnglish (US)
Pages (from-to)383-388
Number of pages6
JournalBiology of the Cell
Volume76
Issue number1
DOIs
StatePublished - 1992

Keywords

  • centrin
  • centrosome
  • cytoskeleton
  • pericentriolar lattice
  • pericentriolar material

ASJC Scopus subject areas

  • Cell Biology

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