TY - JOUR
T1 - Centrin is a component of the periocentriola lattice
AU - Baron, Andre T.
AU - Greenwood, Tammy M.
AU - Bazinet, Christopher W.
AU - Salisbury, Jeffrey L.
N1 - Funding Information:
We thank Mr Mark Sanders and Mr Tien Danh for photographic assistance. This work was supported by NIH grant GM 35258 to JLS and NIH training grant CA 09441 to ATB.
PY - 1992
Y1 - 1992
N2 - Here, we use three polyclonal anticentrin antisera designated 08/28, 26/14-1, and 26/14-2 to further characterize the pericentriolar lattice of metazoan cells. all of these antibodies give an indistinguishable localization pattern that consists of a constellation of pericentrosomal spots. In QT6 cells these spots are few in number and closely associated with the centriolar region, whereas in PtK2 cells they are more numerous and distributed further from the point of microtubule focus. In mitotic cells, centrin is localized to the spindle poles and spindle apparatus. We demonstrate here that the pericentriolar lattice of PtK2 and QT6 cells is, in part, composed of proteins characterized by acidic pIs (4.4 to 5.4), low molecular mass (Mr 18 500-21 000), and calcium-binding; these attributes and the immunoreactivity of these proteins to anticentrin antibodies indicate that they are centrin isoforms of metazoan cells. Finally, we confirm our earlier observation that PtK2 cells contain a centrin-related protein of Mr 165 000; QT6 cells also contain centrin-related proteins (Mr 64 000-165 000). We conclude that centrin is a component of the pericentriolar lattice of higher eukaryotic centrosomes.
AB - Here, we use three polyclonal anticentrin antisera designated 08/28, 26/14-1, and 26/14-2 to further characterize the pericentriolar lattice of metazoan cells. all of these antibodies give an indistinguishable localization pattern that consists of a constellation of pericentrosomal spots. In QT6 cells these spots are few in number and closely associated with the centriolar region, whereas in PtK2 cells they are more numerous and distributed further from the point of microtubule focus. In mitotic cells, centrin is localized to the spindle poles and spindle apparatus. We demonstrate here that the pericentriolar lattice of PtK2 and QT6 cells is, in part, composed of proteins characterized by acidic pIs (4.4 to 5.4), low molecular mass (Mr 18 500-21 000), and calcium-binding; these attributes and the immunoreactivity of these proteins to anticentrin antibodies indicate that they are centrin isoforms of metazoan cells. Finally, we confirm our earlier observation that PtK2 cells contain a centrin-related protein of Mr 165 000; QT6 cells also contain centrin-related proteins (Mr 64 000-165 000). We conclude that centrin is a component of the pericentriolar lattice of higher eukaryotic centrosomes.
KW - centrin
KW - centrosome
KW - cytoskeleton
KW - pericentriolar lattice
KW - pericentriolar material
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U2 - 10.1016/0248-4900(92)90442-4
DO - 10.1016/0248-4900(92)90442-4
M3 - Article
C2 - 1305481
AN - SCOPUS:0026988952
SN - 0248-4900
VL - 76
SP - 383
EP - 388
JO - Biology of the Cell
JF - Biology of the Cell
IS - 1
ER -