Cellular micropatterns with built-in biosensors for detection of extracellular metabolites

J. Yan; J. Y. Lee; S. Shah; A. Revzin

Cellular micropatterns with built-in biosensors for detection of extracellular metabolites

J. Yan, J. Y. Lee, S. Shah, A. Revzin

Physiology & Biomedical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H₂O₂) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.

Original language	English (US)
Pages	1849-1851
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Biosensors
Cell micropatterning
Hydrogel microstructures
Microfabrication

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Cellular micropatterns with built-in biosensors for detection of extracellular metabolites",

abstract = "We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H2O2) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.",

keywords = "Biosensors, Cell micropatterning, Hydrogel microstructures, Microfabrication",

author = "J. Yan and Lee, {J. Y.} and S. Shah and A. Revzin",

year = "2008",

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note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Cellular micropatterns with built-in biosensors for detection of extracellular metabolites

AU - Yan, J.

AU - Lee, J. Y.

AU - Shah, S.

AU - Revzin, A.

PY - 2008

Y1 - 2008

N2 - We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H2O2) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.

AB - We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H2O2) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.

KW - Biosensors

KW - Cell micropatterning

KW - Hydrogel microstructures

KW - Microfabrication

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UR - http://www.scopus.com/inward/citedby.url?scp=84902470079&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902470079

SP - 1849

EP - 1851

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Cellular micropatterns with built-in biosensors for detection of extracellular metabolites

Abstract

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Keywords

ASJC Scopus subject areas

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