Cellular microenvironment modulates the galvanotaxis of brain tumor initiating cells

Yu Ja Huang, Gwendolyn Hoffmann, Benjamin Wheeler, Paula Schiapparelli, Alfredo Quinones-Hinojosa, Peter Searson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Galvanotaxis is a complex process that represents the collective outcome of various contributing mechanisms, including asymmetric ion influxes, preferential activation of voltage-gated channels, and electrophoretic redistribution of membrane components. While a large number of studies have focused on various up- and downstream signaling pathways, little is known about how the surrounding microenvironment may interact and contribute to the directional response. Using a customized galvanotaxis chip capable of carrying out experiments in both two- and three-dimensional microenvironments, we show that cell-extracellular matrix (ECM) interactions modulate the galvanotaxis of brain tumor initiating cells (BTICs). Five different BTICs across three different glioblastoma subtypes were examined and shown to all migrate toward the anode in the presence of a direct-current electric field (dcEF) when cultured on a poly-L-ornithine/laminin coated surface, while the fetal-derived neural progenitor cells (fNPCs) migrated toward the cathode. Interestingly, when embedded in a 3D ECM composed of hyaluronic acid and collagen, BTICs exhibited opposite directional response and migrated toward the cathode. Pharmacological inhibition against a panel of key molecules involved in galvanotaxis further revealed the mechanistic differences between 2- and 3D galvanotaxis in BTICs. Both myosin II and phosphoinositide 3-kinase (PI3K) were found to hold strikingly different roles in different microenvironments.

Original languageEnglish (US)
Article number21583
JournalScientific Reports
Volume6
DOIs
StatePublished - Feb 22 2016
Externally publishedYes

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Cellular Microenvironment
Neoplastic Stem Cells
Brain Neoplasms
Electrodes
Extracellular Matrix
Myosin Type II
1-Phosphatidylinositol 4-Kinase
Laminin
Hyaluronic Acid
Glioblastoma
Collagen
Stem Cells
Pharmacology
Ions
Membranes

ASJC Scopus subject areas

  • General

Cite this

Cellular microenvironment modulates the galvanotaxis of brain tumor initiating cells. / Huang, Yu Ja; Hoffmann, Gwendolyn; Wheeler, Benjamin; Schiapparelli, Paula; Quinones-Hinojosa, Alfredo; Searson, Peter.

In: Scientific Reports, Vol. 6, 21583, 22.02.2016.

Research output: Contribution to journalArticle

Huang, Yu Ja ; Hoffmann, Gwendolyn ; Wheeler, Benjamin ; Schiapparelli, Paula ; Quinones-Hinojosa, Alfredo ; Searson, Peter. / Cellular microenvironment modulates the galvanotaxis of brain tumor initiating cells. In: Scientific Reports. 2016 ; Vol. 6.
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