Cell labeling and targeting with superparamagnetic iron oxide nanoparticles

Brandon J. Tefft, Susheil Uthamaraj, J. Jonathan Harburn, Martin Klabusay, M. (Dan) Dragomir Daescu, Gurpreet S. Sandhu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Targeted delivery of cells and therapeutic agents would benefit a wide range of biomedical applications by concentrating the therapeutic effect at the target site while minimizing deleterious effects to off-target sites. Magnetic cell targeting is an efficient, safe, and straightforward delivery technique. Superparamagnetic iron oxide nanoparticles (SPION) are biodegradable, biocompatible, and can be endocytosed into cells to render them responsive to magnetic fields. The synthesis process involves creating magnetite (Fe3O4) nanoparticles followed by high-speed emulsification to form a poly(lactic-co-glycolic acid) (PLGA) coating. The PLGA-magnetite SPIONs are approximately 120 nm in diameter including the approximately 10 nm diameter magnetite core. When placed in culture medium, SPIONs are naturally endocytosed by cells and stored as small clusters within cytoplasmic endosomes. These particles impart sufficient magnetic mass to the cells to allow for targeting within magnetic fields. Numerous cell sorting and targeting applications are enabled by rendering various cell types responsive to magnetic fields. SPIONs have a variety of other biomedical applications as well including use as a medical imaging contrast agent, targeted drug or gene delivery, diagnostic assays, and generation of local hyperthermia for tumor therapy or tissue soldering.

Original languageEnglish (US)
Article numbere53099
JournalJournal of Visualized Experiments
Volume2015
Issue number104
DOIs
StatePublished - Oct 19 2015

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Keywords

  • Biodegradable
  • Bioengineering
  • Capture
  • Delivery
  • FeO
  • Ferrofluid
  • Issue 104
  • Magnetic
  • Magnetite
  • Paramagnetic
  • PLGA
  • Sorting
  • SPION

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Tefft, B. J., Uthamaraj, S., Harburn, J. J., Klabusay, M., Dragomir Daescu, M. D., & Sandhu, G. S. (2015). Cell labeling and targeting with superparamagnetic iron oxide nanoparticles. Journal of Visualized Experiments, 2015(104), [e53099]. https://doi.org/10.3791/53099