Cell Elasticity Determines Macrophage Function

Naimish R. Patel, Medhavi Bole, Cheng Chen, Charles C. Hardin, Alvin T. Kho, Justin Mih, Linhong Deng, James Butler, Daniel J Tschumperlin, Jeffrey J. Fredberg, Ramaswamy Krishnan, Henry Koziel

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different origins and species, we report that macrophage elasticity is a major determinant of innate macrophage function. Macrophage elasticity is modulated not only by classical biologic activators such as LPS and IFN-γ, but to an equal extent by substrate rigidity and substrate stretch. Macrophage elasticity is dependent upon actin polymerization and small rhoGTPase activation, but functional effects of elasticity are not predicted by examination of gene expression profiles alone. Taken together, these data demonstrate an unanticipated role for cell elasticity as a common pathway by which mechanical and biologic factors determine macrophage function.

Original languageEnglish (US)
Article numbere41024
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 18 2012
Externally publishedYes

Fingerprint

Macrophages
Elasticity
elasticity (mechanics)
macrophages
cells
Biological Factors
Substrates
Transcriptome
Gene expression
Polymerization
polymerization
Rigidity
actin
Ligation
Actins
homeostasis
particulates
Homeostasis
inflammation
Chemical activation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Patel, N. R., Bole, M., Chen, C., Hardin, C. C., Kho, A. T., Mih, J., ... Koziel, H. (2012). Cell Elasticity Determines Macrophage Function. PLoS One, 7(9), [e41024]. https://doi.org/10.1371/journal.pone.0041024

Cell Elasticity Determines Macrophage Function. / Patel, Naimish R.; Bole, Medhavi; Chen, Cheng; Hardin, Charles C.; Kho, Alvin T.; Mih, Justin; Deng, Linhong; Butler, James; Tschumperlin, Daniel J; Fredberg, Jeffrey J.; Krishnan, Ramaswamy; Koziel, Henry.

In: PLoS One, Vol. 7, No. 9, e41024, 18.09.2012.

Research output: Contribution to journalArticle

Patel, NR, Bole, M, Chen, C, Hardin, CC, Kho, AT, Mih, J, Deng, L, Butler, J, Tschumperlin, DJ, Fredberg, JJ, Krishnan, R & Koziel, H 2012, 'Cell Elasticity Determines Macrophage Function', PLoS One, vol. 7, no. 9, e41024. https://doi.org/10.1371/journal.pone.0041024
Patel NR, Bole M, Chen C, Hardin CC, Kho AT, Mih J et al. Cell Elasticity Determines Macrophage Function. PLoS One. 2012 Sep 18;7(9). e41024. https://doi.org/10.1371/journal.pone.0041024
Patel, Naimish R. ; Bole, Medhavi ; Chen, Cheng ; Hardin, Charles C. ; Kho, Alvin T. ; Mih, Justin ; Deng, Linhong ; Butler, James ; Tschumperlin, Daniel J ; Fredberg, Jeffrey J. ; Krishnan, Ramaswamy ; Koziel, Henry. / Cell Elasticity Determines Macrophage Function. In: PLoS One. 2012 ; Vol. 7, No. 9.
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